110 



-19^1- 



fractlon (l6 per cent), a soluble nitrogenous material (from 

 10 to 20 per cent) and a small amount of lipid » The poly- 

 saccharide was highly dextro-rotatory, ketose and pentose- 

 free, but contained d -galactose, d-mannose and d -glucose. 

 There were no uronlc acid residues. Identical polysaccharides 

 have been prepared from the dried organisms directly. The 

 polypeptide component was insoluble in water, soluble in 

 dilute alkalis, and contained 8o2 per cent N, partly as tyro- 

 sine and arglnine units o Approximately 50 per cent of the 

 total nitrogen appeared as free amino -groups after acid hydrol- 

 ysis. The nature of the soluble nitrogenous component was 

 not determined. 



320. GOLDRING, W., CHASIS, H. ^RANGES, H. A., and SMITH, H. W. 



Effective renal blood flow in subjects with essential hyperten- 

 sion 



J. Clin, Invest o 20:637-653, 19^1 



Filtration rates, diodrast clearance, and maximal rate of tubu- 

 lar excretion of diodrast were determined in 6o patients with 

 essential hypertension. The results were compared with those 

 obtained from a previous study of the normal kidney. The 

 effective blood flow per unit of functional renal tubular tissue 

 was usually below normal, the filtration fraction elevated, and 

 the maximal rate of diodrast excretion far below normal. 

 Small doses of pyrogenic Inulin resulted in renal hyperemia and 

 a fall in the filtration fraction. In some patients, the 

 maximal rate of diodrast excretion in the tubule increased 

 during hyperemia. 



321. HARTWELL, J. Lo, SHEAR, M. J,, KAHLER, H. and TURNER, P. C. 



Properties of the fraction of B. prodigiosus which produced 

 hemorrhage in mouse sarcomas 



Cancer Res. l:74l, 1941 



A summary is given of the methods now used to determine the 

 properties of the hemorrhage -producing filtrate concentrate 

 of Shear. Protein content was below 1 per cent; the biuret, 

 ninhydrln, xanthoproteic and Millon's tests were negative. 

 High molecular weight components were present as shown by dia- 

 lysis of the filtrate. The Molisch reaction was positive, 

 while iodine-iodide and Benedict tests gave negative results. 

 Freezing of active solutions destroyed most of the potency. 

 All fractions appeared to be equally toxic to tumor tissue when 

 a bioassay was made on mice bearing growing sarcomas. Two 

 active fractions were obtained by ultra-centrif ugation and 

 electrophoresis . 



