Ill 

 -19^1- 



322. MACLEOD, C. M, and AVERY, Oc T. 



The occurrence during acute Infections of protein not normally 

 present in the blood „ II, Isolation and properties of the 

 reactive protein 



J, Exper. Med. 73:183-190, 19^1 



The reactive protein present In the serum albumin fraction of 

 blood and precipltable by the C polysaccharide of pneumococci^ 

 becomes soluble in tap-water after removal of the alcohol- 

 ether soluble lipids and is no longer precipltable by calcium 

 in small amounts c It still retains capacity for precipitation 

 with the C polysaccharide « 



323. MORGAN, Ho R. 



Immunologic properties of an antigenic material Isolated from 

 Eberthella typhosa 



J, Immunol e ^I:l6l-l80, 19^1 



The py-rexlal substance from E„ typhosa is antigenic and elicits 

 fever In the animal when circulating antibodies are present. 

 Washed precipitates of antigen and excess antibody cause ele- 

 vations in temperature and leucopenla. Leukocytosis follows. 

 The factor is heat-stable c Activity remains in the acid- 

 insoluble portion after acid hydrolysis. 



324. MORGAN, W. To J, and PARTRIDGE, S. M. 



S todies in Immunochemistry, VI. The use of phenol and of 

 alkali in the degradation of antigenic material isolated from 

 Bact . dysenterlae ( Shiga ) 



Blochem. J. 35:ll40-ll63, 19^1 



The antigenic complex of B, dysenterlae Shiga Is soluble in 90 

 per cent phenol solution^, partially soluble In aqueous alkaline 

 solution. The 'smooth' variant yields the antigenic complex 

 and nonspecific water-insoluble simple proteins, the 'rough' 

 variant yields no antigenic material. Polysaccharide compo- 

 nents can be isolated in a free state by dialysis of the phenol 

 solution o This protein-free carbohydrate fails to Induce formation 

 of specific 'Shiga' agglutinins or precipitins in the rabbit. 

 The acidic (polypeptide-llke) protein component appears to be 

 a conjugated protein, non-antlgenic when separated from the 

 polysaccharide, but, on recombination with undegraded specific , 

 polysaccharide, a powerful antigen. A simple protein isolated 



