128 



i 



- 1 9 If 3 - 



370. HARRISON, Jo W,^ MYERS, Re J, and HART, D, So 



Purified mineral-free water for pharmaceutical purposes 



Jo Am. Pharra. Ao 32:121-128, 1943 



By the use of Ion exchange resins, pyrogens In mineral-free 

 water or? In solutions- Intended for parenteral use may be re- 

 duced. The amount of pyrogenic material remaining does not 

 produce temperature increases above 0.5° C. 



371. HARTWELL, J. Lo, SHEAR, Mo J. and ADAMS, J. H., JTl. 



Chemical treatment of tumors. VII. Nature of the hemorrhage- 

 producing fraction from Serratia mareescens ( Bacillus prodigiosus ' 



Jo Nat. Cancer Inst. 4:107-122, 1943 



The active material contained 47.5 per cent C, ,7.1 per cent H, 

 2.2 per cent N, 1.1 per cent P and 2.2 per cent acetyl. Ash 

 was 3.5 per cent. The minimal hemorrhage -producing dose contains 

 about Ool gamma of total solids. Neither protein nor poly- 

 peptide can be demonstrated after hydi^olysis; hexosamlne, 

 methylpentose and a firmly bound phosphoiipld are present. 

 Although stable at room temperatures, the potency is lost at 

 increasing rates as temperatures increase between 60° C. and 

 100° Co 



372. HARTWELL, J. L., SHEAR^ M. J. and ADAMS, J. R., JR. 



Nature of the bacterial polysaccharide which produces hemorrhage 

 in mouse sarcoma 



Cancer Res. 3^122, 1943 



"Further data were given on the material obtained from cultures 

 of Bo prodigiosus " o The polysaccharide fraction had previously 

 given negative results in tests for protein; in this study 

 colorlmetric tests for tyrosine, arglnlne and hlstldlne gave 

 positive results. The active agent could be separated from 

 the protelnate material by tryptic digestion o Potency of the 

 active portion disappeared when the acetyl content was lost during 

 treatment with 0.1 N/i^aOH« By use of 0.1 N/MaHC03, the acetyl 

 was removed with Injury to activity. Hydrolysis (1.5 N/HCl) pro- 

 duced 60 per cent reducing sugars and 16 per cent of a water-in- 

 soluble, alkali-soluble product containing N and P. The re- 

 ducing sugar was approximately 30 per cent methylpentose. * 



