129 



-19^3- 



Gluccsamine was about k per cent. No ketose or desoxy- sugars 

 were noted; galactose, mannose and Inositol were not present, 

 A small amount of uronic acid was measured. The end-point 

 dose (producing hemorrhage in 50 per cent of tumors) v^?as about 

 0.1 gamma. 



573. HUTNER, S. H, and ZAHL, P. A. 



Action of bacterial toxins on tumors. IV. Distribution of 

 tumor-hemorrhage agents among bacterial species 



Proc. Soc. Exper. Biol. Med e 52:364-368, 1943 



When 30 gram-negative and 7 gram-positive killed organisms, 

 pathogenic or non-pathogenic, were injected in doses approach- 

 ing lethal limits into mice bearing mouse sarcoma l80, repre- 

 sentatives of gram-negative species induced tumor hemorrhage 

 (with few exceptions). But one gram-positive bacterium 

 ( Listeria monocytogenes ) possessed the capacity to produce 

 vascular stasis in tumors. It is argued that tumor -hemorr- 

 haging toxins may be bound in the somatic antigens, charac- 

 teristic of gram-negative organisms. 



574. KAHLER, H., SHEAR, M. J. and HARTWELL, J. L. 



Chemical treatment of tuitiO]?s. VIII. Ultracentrif ugal and 

 electrophoretic analysis of the hemorrhage-producing fraction 

 from Serratia marcescens ( Bacillus prodigiosus ) 



J. Nat. Cancer Inst. 4:123-129, 1943 



Analyses of 3 preparations of the polysaccharide fraction 

 from S. marcescens which produces tumor hemorrhage gave the 

 following information: the non-dialyzable solids were approx- 

 imately 1 per cent of weight o The partial volume of poly- 

 saccharide by ultracentrifugal analysis was 0.75; the sedimen- 

 tation rates differed. No polysaccharide component appeared 

 to carry potency for other activities than that of producing 

 hemorrhage in neoplasms. This potency was about equally divided 

 between sediment and supernatant fluid. The electron micro- 

 graph revealed that the active principle was a large symetrical 

 particle with molecular weight somewhere between 3 and 30 million. 

 It appears to remain in the material most easily sedimentable. 

 The potency is lost rapidly at ordinary temperatures and pressures 



