132 



-19 4 3- 



381. SHEAR, M. J. and TTJRNER, P. C. 



Chemical treatment of tumors. V. Isolation of the hemorrhage- 

 producing fraction from Serratia marcesc ens (Bacillus prodieio- 

 sus) culture filtrate ^ ^ — 



J. Nat. Cancer Inst. 4:8l-97, 19^3 



Highly potent preparations of the active agent for tumor hem- 

 orrhage were prepared from filtrates of ^. marcescens through 

 precipitation with chloroform, ethyl alcohol and dialysis. 

 The active fractions were tested for potency, chemical prop- 

 erties and toxicity. The carbohydrate components were in the 

 majority (positive Molisch) and the protein very small or ab- 

 sent (negative biuret, trichloracetic acid and sulfosalicylic 

 acid). pH changes between 3.0 and 10.0 did not affect potency. 

 Lyophilization produced no loss of activity. The most active 

 concentrate obtained had a potency of 130,000 m.t.u. per cc. 

 The lethal dose ranged from 100 to 1000 times the minimum 

 hemorrhagic dose (0.1 gamma total solids). Fractions of low 

 potency were eliminated through a bioassay technique. When 

 tumors were treated early in growth, they did not respond to 

 bacterial action; old tumors were prone to develop brown dis- 

 colorations which obscured hemorrhage. 



382. TAYLOR, R. D. and PAGE, I. H. 



Severe renal irritation resulting from foreign protein (typhoid) 

 fever therapy <= *- \ jf 1 



J.A.M.A. 121:754-755, 1943 



Severe renal irritation developed during treatment with intra- 

 venous typhoid vaccine of patients ill with malignant hyperten- 

 sion and Bright 's disease with nephrosis. Caution must be 

 employed in the use of this type of therapy where renal disease 

 exists, 



383. WELCH, H., CALVERY, H. 0., McCLOSKY, W. T. and PRICE, C. W. 

 A method of preparation and test for bacterial pyrogens 



J. Am. Pharm, A. 32:65-69, I943 



Lack of antigenicity in Pseudomonas aeruginosa pyrogen was shown 

 by the fact that, in a test in rabbits, ll injections were made 

 in each animal over a period of 9 weeks without tolerance to 

 the dosage developing. 



