137 



-19 4 4- 



subcutaneous doses of the polysaccharide of B. prodlglosus 



ranging from 1 to 8 mg. per kg. of body weight In the dog^and 

 5.0 to 10.0 mgo (subcutaneous administration only) per kg. in 

 the rat. Adrenalin was administered to certain animals in an 

 attempt to prevent blood pressure depression. 



Death from circulatory failure occurred in the anesthetized 

 dog 1 to 4 hours after the administration of 0.3 to 4.3 mg. 

 per kg. of body weight of the polysaccharide. Adrenaline was in- 

 effective for restoration or maintenance of the blood pressure. 

 In the unanesthetized dog, death occurred in some 12 to 24 hours 

 after the administration of amounts between 1 and 8 mg. Necropsy 

 revealed extensive intestinal congestion and hemorrhage. 

 Subendocardial hemorrhage was also noted. Capillary permeability 

 in rats and fragility in dogs approached the normal. Rise in 

 body temperature and increased NPN were recorded for all animals. 



PRANKE, F. E. 



Attempts to produce anaphylaxis in guinea pigs with the poly- 

 saccharide from Serratia marcescens ( Bacillus prodlglosus ) 



J. Nat. Cancer Inst. 5:173-177, 1944 



Sensitizing doses of a lyophilized preparation of the poly- 

 saccharide from S. marcescens (P-2l) and constant shocking doses 

 of the material did not produce a typical picture of anaphylaxis 

 in the guinea pig. 



PRANKE, P. E. and RICHERT, D. 



Effects of sublethal doses of a polysaccharide from Serratia 

 marcescens ( Bacillus prodlglosus ) on the electrocardiogram, 

 blood ascorbic acid and non-protein nitrogen of the dog 



J, Nat. Cancer Inst, 5:179-183, 1944 



Electrocardiographic studies of the dog, following the injection, 

 subcutaneously or into the peritoneum, of 3.0 to 5.7 mg. per 

 kg. of body weight of the polysaccharide from S. marcescens , 

 revealed no significant changes. Development of a sinus tachy- 

 cardia was noted. Four of the animals exhibited a rise in 

 blood ascorbic acid, ranging from 0=98 to 1.86 rag. per cent. 

 The Increase in non-protein nitrogen ranged from 44 to 6l.7 tng. 

 per cent. There was considerable gastrointestinal congestion 

 and hemorrhage. Only mild intoxication vjas noted in two 

 animals. Serum calcium was within normal limits. 



