140 



-1944- 



403. ROBINSON, E. S. and FLUSSER, B. A. 



Studies on pyrogens. The Isolation of pyrogens from various 

 microorganisms 



J. Biol, Chem, 153:529-534, 1944 



Precipitates from suspensions of typhoid vaccine, Proteus 

 vulgaris and Pseudomonas aeruginosa cultures, after extraction 

 with 95 per cent phenol, dialysis and repreclpltatlon with 

 acetone, contain no nitrogen. Ash remains (4 to 12 per cent). 

 Reducing sugars are found only after hydrolysis. The pyrogen 

 from typhoid vaccine is homogeneous, with a molecular weight 

 of approximately 62,000. 



The minimal pyrogenlc dose for the rabbit has been found to be 

 0.5 gamma per kg, for the preparation from E. typhosa , 2.0 gammas 

 per kg. for that from Ps. aeruginosa , and 1.0 gamma for that 

 from Proteus vulgaris . The maximal temperature Increase for 

 typhoid pyrogen occurred when 25 to 50 gammas per kg. were 

 Injected. 



404. ROSA, M, C. and de ARAGAO, R. M. 



Do plrogenio (Pjrrogen) 



Hospital (Rio de Janeiro) 25:201-211, 1944 



The occurrence of pyrogens in aqueous solutions is discussed 

 with specific reference to teating methods. Variations in 

 body temperature in test animals may have spontaneous genera- 

 tion unrelated to pyrogens, 



405. SHWARTZMN, G, 



Phenomenon of local skin reactivity to Serratla marcescens 

 ( Bacillus prodlglosus ) , Immunological relationships between 

 Serratla marcescens culture filtrates and Shear polysaccharide 



Cancer Res. 4:191, I944 



The Shear polysaccharide and S^ marcescens culture filtrates 

 are closely related in tumor hemorrhage and necrosis-inducing 

 properties. Serologic cross-precipitation of rabbit Immune 

 sera with the two substances and cross neutralization of 

 provocative factors showed close antigenic relationships 

 exist between the two, with a somewhat heightened antigenicity 

 residing in the culture filtrate. 



