- 1 9 4 5 - 



J. Clin. Invest. 24:749-758, 19^5 



Eight subjects, 6 of whom had essential hypertension and 2 

 controls with normal blood pressure held at basal levels 

 (bed rest), received premedication with amidopyrine during 

 the 24 hours immediately preceding pyrogen administration. 

 Contaminated inulin and typhoid vaccine were used as pyrogenic 

 agents. Cardiac output, arterial pressure, renal plasma 

 flow and glomerular filtration rats were determined. 



1 



In the normal subjects, pyrogen administration produced increased 

 cardiac output, decreased peripheral resistance and hypotension. 

 Two hypertensive subjects exhibited blood pressure reductions 

 to the normal range; in a third, hypotension was extreme and 

 required special measures (Trendelenburg position); for all 

 hypertensive subjects, hypotension to shock levels was noted 

 in extremities. All subjects had high degrees of renal 

 vasodilatation. 



421. CAMPBELL, D. H. and GHERKIN, A. 



The destruction of pyrogens by hydrogen peroxide 



Science, 102:535-536, 1945 



Pyrogenic solutions of gelatin were rendered nonpyrogenic by 

 heating with KMn04 or H2O. Where the oxidation does not affect 

 other constituents of the solution, the method may be applied, 

 provided no harmful excess remains. 



422. CAMEJO, E. G. 



PirogenOs ?n las solutions inyectables (Pyrogens in injectable 

 solutions 



Escuela farm. 8:11, 1945 



Pyrogens may be caused by the presence of killed bacteria, by 

 bacterial proteins or they may be 'microscopic particles'. 

 Although they may be eliminated in most cases by activated 

 charcoal filtration, only the biologic test gives conclusive 

 proof of their presence or absence in pharmaceutical solutions. 



