148 



-19^5- 



427. MENKIN, V. 



Chemical basis of fever with inflammation 



Arch. Path. 39:28-36, 19^5 



'Pyrexin' a temperature-elevating fraction, has been secured 

 by enzyme action in 'necrosin' the toxic substance present 

 In the euglobulln of inflammatory exudates. The exudate, its 

 fractions and 'necrosin' were injected intravenously into rabbits 

 and compared with changes noted in rectal temperatures after 

 injection of physiologic saline solution. The whole exudative 

 material produced increases, regardless of the amount adminis- 

 tered, averaging 2o37'^ Po Neither pseudoglobulin or albumin 

 fractions were pyrexial. Necrosin, the euglobulin fraction, 

 produced maximal increases (to 4. 4° P.) within 6 hours, or a 

 276 per cent increase. The active portion of necrosin alone 

 produced a 290 per cent increase in temperature. Nonhemolyzed 

 canine blood serum increased temperature 67 per cent; hemolyzed 

 blood brought these figures to 209.5 per cent. The injury 

 to blood cells may release a pyrogenic factor and this also 

 explains the fever-activity of malarial therapy and trauma, 



'Pyrexln' has no proteolytic activity but may be released throu^ 



such processes. Purified necrosin brings rapid liquefaction of 



thromboses. It yields the fever-producing agent, stable to 



heat, inactive after ashing. The latter is insoluble in ether,' 



95 per cent alcohol and concentrated sulfuric acid, distilled 



water, sodium chloride and ammonium sulfate, but is soluble 



in weak alkalis. In various samples, the nitrogen content 



ranged between 10.23 and 12.93 per cent, phosphorus from 



0.79 to 1.39 per cent, ^t was not hydrolyzed by trypsin. The 



Molisch reaction was negative, the ninhydrin positive. It 



may be a peptide linked to a nucleic acid derivative. The 



mode of action shown by the substance Is one of stimulation 



of the fever control centers of the hypothalamus. Its activity 



is reduced by salicylates and barbiturates, 



428. MEYER, S. R. 



Indiclos da exlstencla de una substancia antineoplasica formade 

 mas liquldos de cultura de Penlc 1111 urn notatum (Evidence for 

 the existence of an ant l-neopIa^tic~sub stance as a metabolite 

 from Penicillium notatum culture fluids) 



Arq, Inst. biol. (Sao Pauls') 16:307-314, 1945 



