-19^5- 



^29. PROBEY, T. P. and PITTMAN, M. 



The pyrogenlcity of bacterial contaminants found in biologic 

 products 



J. Bact. 50:397-411, 19^5 



In the preparation of human blood and plasma, gram-positive 

 and gram-negative bacilli were isolated. Most pyrogenic of 

 these were gram-negative cocci, B. subtilis , certain coryne- 

 bacterium, colon and Pseudomonas groups. Gram-positive 

 organisms were 100 to 1000 times less pyrogenic than gram-nega- 

 tive organisms; the more usual reaction to the injection of such 

 organisms was a drop in temperature. Blood serum culture 

 medium appeared to produce organisms yielding more pyrogen. 

 Autoclaving for 15 minutes did not destroy pyrogenicity, but, 

 in the gram-positive bacilli preparations, such drastic treat- 

 ment markedly reduced it. 



^30. RODNEY, G. and WELCKE, M. 



Studies on bacterial pyrogens (Abstract) 



J. Bact. 50:129, 19^5 



Bacterial pyrogens from various strains of Ps. aeruginosa , 

 E. typhosa and B. subtilis contained 2 per cent glucosamine 

 7rom which a large proportion of reducing sugars (32 per cent) 

 could be released. All were hexoses; galactose, fructose 

 and uronic acid were not found. Thymus nucleic acid was closely 

 bound to the hexose. Hydrolysis did not follow treatment with 

 lysozyme, diastates and pancreatic enzymes. The pyrogens did 

 not appear to increase cell oxidation, although they caused 

 body temperature in animals to rise. 



;i. SMITH, D. C, SHAPER, J. C. and CRUTCHPIELD, A. J. 



Pever therapy with intravenous foreign protein in neurosyphilis 



Southern Med. J. 38:3; 19^, 19^5 



'With the method here described, we have available a method for 

 producing prolonged high fever, foreign protein reaction, free- 

 dom from the untoward reactions associated with malaria and an 

 opportunity to intensify chemotherapy' . 



