158 



-19^6- 



453i OLITZKI, Lo, CZACZKES, J. W. and KUZENEK, A. 



Endotoxlc factors of Rickettsia Frowazekl and their Immunological 

 relationship to the endotoxins of other gram-negative micro- 

 organisms 



J. Immunol. 53:365-370, 19^6 



Hypothermia and decreased liver glycogen follows the Intrab- 

 domlnal Injection of heat-killed Rickettsial organisms. To 

 determine whether or not Immunologic relationships exist between 

 these factors In R„ Frowazekl and In certain other gram-negative 

 bacteria ( Prote^a , S. dysenterlae ) and two gram-tjosltlve bacteria, 

 Corynebacterlum erose and Mycobacterium butyrlcum . Any of the 

 three gram-negative organisms elicited Immunization against hypo- 

 thermia and decreased liver glycogen produced by either of the 

 other two. This Is conceded to be "a specific Immune reaction 

 directed against a toxic product which Is a common constituent 

 of a large group of microorganisms." The gram-posltlve organisms 

 used did not elicit hypothermia or Immunity against temperature 

 reduction. 



454. OLITZKI, L., SHELUBSKY, M. and HESTRIN, S. 



Pathogenlzlng effect of different carbohydrates on Eberthella 

 typhosa 



Proc. Soc. Exper. Biol, Med, 63:491-496, 1946 



In determining the chemical basis for the pathogenlzlng activity 

 of certain carbohydrates, mice were Injected with the test 

 organism (E_^ typhosa ) Intraperltoneally and with a sterile aqueous 

 solution of the tested pathogenlzlng substance In varying concen- 

 trations. By this method 15 polysaccharides and 11 simple sugars 

 and oligosaccharides were examined. Three classes of carbohy- 

 drates were distinguished: (1) powerful pathogenlzlng sugars, 

 levan, dextran, mucin and agar;; (2) moderate pathogenlzlng In two 

 cellulose oreparatlons. In gum acacia, glycogen, mannan, and 

 Inulln; (3) non-pathogenizlng crystalline sugars and the starch 

 and galac to -mannan of the polysaccharides. Pathogenlzlng ability 

 was enhanced by addition of kaolin to the solution injected. 

 The pathogenicity of levan, produced in vitro , may be an intrinsic 

 property. It is suggested that specific colloidal patterns, rather 

 than polar groupings or individual unit structure, may underlie 

 pathogenlzlng activity. 



455. PAINE, T. P. 

 Illness in man following inhalation of Serratia marcescens 



