159 



-19^6- 

 J. Infect. Dis. 79:226-232, 1946 



Pour adult males, two of whom had histories of previous exposure, 

 were subjected to an aerosol concentration of S. marcescens of 

 approximately 2 million per cubic foot of air for two and one- 

 half hours. Blood, nasal secretion and sputum cultures were 

 made, serum agglutinins determined and blood changes recorded. 

 Two and one-half to four hours after the exposure all subjects 

 became acutely ill. Ocular and respiratory symptoms appeared. 

 Chills and fever, tachycardia and generalized myalgias were 

 present. Subjective improvement appeared after diaphoresis 

 and defervescence. No significant changes in blood or urine 

 components were noted, but sputum and nasal secretions produced 

 positive cultures. Marked neutrophilia appeared at the onset of 

 the reaction. Specific serum agglutinins were present in high 

 titer. Previous exposure appears to be immunologically significant 



456. REIMANN, S. P., HOLLOMAN, A. L., OAKEY, R., KcCONNELL, J. R. 

 and SHEAR, M. J. 



Gross and microscopic pathology of human tumors following adminis- 

 tration of S. marcescens polysaccharide 



Cancer Res. 6:489, 1946 (Abstract) 



Without previous sensitization^ the injection of a polysaccharide 

 prepared from B. prodlgiosus produces necrosis and hemorrhage in 

 both animal anB" human tumors. Intravenous injection of the poly- 

 saccharide in a single dose or in divided doses, was followed in 

 a few hours by swelling in the tumor area. For several patients, 

 a constant Intravenous drip of the polysaccharide in solution was 

 used and, for others, intraperitoneal Injections. Diffuse 

 ecchsrmosis appeared within 48 hours in tumors near the surface. 

 The size of the tumor is believed to be a determining factor in 

 Judging the amount of necrosis and hemorrhage produced by a 

 given dosage. Small neoplasms showed more extensive necrotic 

 changes. Microscopically, the hemorrhages were seen to be focal 

 in most cases, and the necrosis was of the liquefaction type. 



The distinction between hemorrhage from polysaccharide and that 

 occurring spontaneously in softer sarcomas is often a finely 

 drawn one. Fibrosarcomas show little or no hemorrhage, hence 

 presence of these changes after polysaccharide injection may be 

 considered a positive confirmation of polysaccharide activity. 



^57. TODD, J. P., MILNE, G. R. and LAURIE, J. P. 

 Non-pyrogenic saline infusion fluids 

 Chem. and Druggist 145:536, 1946 



