l69 



- 1 9 4 7 - 



Fractionation of the polysaccharide from one strain of S. mar - 

 c esc ens has yielded a product which appears to be less ^oxTc" 

 and antigenic In the mouse. No significant alteration of the 

 tumor-necrotlzlng property occurred. Studies have been made of 

 the protection afforded by passive Immunization of mice against 

 the toxic fraction of the polysaccharide from the "GW" strain. 

 The gamma globulin fraction of rabbit antlsera, bearing anti- 

 bodies, protected a large percentage of the animals when In- 

 jected a few hours before the Injection of a lethal dose of 

 polysaccharide. Previous Injection of the fraction Into mice 

 bearing sarcoma 37 prevented toxic effects of a large dose of 

 S, marcescens polysaccharide but did not Interfere with tumor 

 necrosis, A~straln of S. marcescens (724) has been found to 

 be capable of producing less toxic polysaccharides. 



485. DILLER, I. C. 



Degenerative changes Induced In tumor cells by Serratl a mar - 

 cescens polysaccharide 



Cancer Res. 7:605-626, 1947 



A detailed account of the cellular changes Induced In tumors 

 by the polysaccharide of S. marcescens Is presented. Studies 

 were carried out on mouse sarcoma 37, on spontaneously appearing 

 rat and mouse carcinoma and on human biopsy material taken from 

 16 carcinoma patients. The combined effects of polysaccharides 

 and roentgen therapy on neoplastic destruction were observed. 



Two effects of polysaccharide activity were noted; one, a vas- 

 cular change, the other, one of nuclear destruction. Maximal 

 damage was seen within six hours. There was marked Inhibition 

 of resting cell division for three days after treatment. In 

 some animals no viable cells were detected three days after 

 treatment with a dose lethal for one-fifth to one-third of the 

 mice. Roentgen therapy Increased three-fold the number of 

 tumors which showed regression. Injection dlr»ectly Into the 

 tumor did not Inhibit growth. Resistance to the necrotizing 

 effects of polysaccharide developed In the tumor host after 

 repeated Injections, Human tumors underwent similar but less 

 pronounced destructive changes, 



486. DILLER, I. C. 



The effect of simultaneous administration of bacterial poly- 

 saccharide and adrenal cortex extract on cells of mouse tumors 

 and on the adrenal glands of the host 



Cancer Res. 7:715, 1947 (Abstract) 



The action of the polysaccharide of S. marcescens on sarcoma 37 



