182 



-19^8- 



evaluated by histologic examination of tumor tissue following 

 administration of 10 or 20 gammas of the preparations into 

 Swiss mice bearing transplanted Sarcoma 37 • 



Toxicity and antigenicity were more pronounced in P3 type poly- 

 saccharides, while less variation between the three types was 

 evident in their effect on hemorrhage and necrosis production 

 in tumors. Serologically the polysaccharides from two strains of 

 S. marcescens differed. Some fractionation products were but 

 weakly antigenic and relatively nontoxic but retained tumor- 

 necrotizing properties. Storage for a period of years reduced 

 anti-neoplastic qualities but altered toxicity and antigenicity 

 very little. Freezing appeared to enhance the tumor-necro- 

 tizing acitivity. 



520. CREECH, H, J,, HAMILTON, M. A,, NISHIMURA, E, T. and HANKWITZ, 

 R , F. , JR « 



The influence of antibody containing fractions on the lethal 

 and tumor-necrotizing actions of polysaccharide from Serratia 

 marcescens ( Bacillus prodigiosus ) 



Cancer Res. 8:330-336, 19^3 



Normal mice and those bearing dorsal transplants of Sarcoma 37 

 were passively immunized with gamma globulin fractions isolated 

 from rabbit serum after injection of polysaccharides produced 

 by two strains of S. marcescens . Both antisera and gamma glob- 

 ulin fractions projected mice against lethal doses of poly- 

 saccharide from the same strain of S, marcescens but not against 

 that of a heterologous strain. One type of antisera and gamma 

 globulin gave no protection against homologous polysaccharide. 

 Lethal acitivity of one polysaccharide was decreased by use of 

 the specific gamma globulin fraction. The reduction of lethality 

 did not affect tumor hemorrhage production in the mouse, 



521. DELAUNAY, A,, BOQUET, P., LEBRUN, J., LEHOULT, Y. and DELAUNAY, 

 M. 



The mode of action of bacterial endotoxins, IV. The vasomotor 

 disturbances and their consequences in the intoxicated animals, 



J. Physiol. 40 J 89, 19^8 



The mesenteric vessels of guinea pigs and mice and the cutaneous 

 vessels of the rabbit ear were examined microscopically after 

 a lethal dose of S, typhi antigen. A few minutes after injection 

 of the antigen, irregular contractions of arterioles and medium- 

 sized arteries were seen, with weaker contractions affecting the 

 venous side. Dilatation of the vessels occurred in the terminal 

 stages. Throughout intoxication, diapedesis of leukocytes was 

 inhibited. 



