188 



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537. LEWIS, L. A. and PAGE, I. H. 



Changes In blood leukocyte level of adrenalectotnlzed and normal 

 rats following administration of typhoid vaccine 



Am. J. Physiol. 153:148-152, 19^8 



Adrenalectomized rats were maintained on either adrenal extract, 

 dehydrocorticosterone acetate or physiologic saline solution 

 for three days, at which time 1.33 MLD of typhoid vaccine was 

 administered intraperitoneally. Determination of blood leuko- 

 cyte and differential levels and of hemoglobin followed. Results 

 were compared with values found in normal animals and humans 

 after immunizing doses of typhoid vaccine. 



Blood leukocyte values changed little after injection of adrenal 

 extract, compound A acetate or saline, but marked lymphopenia 

 and neutrophilia were noted after typhoid vaccine injections. 

 These were enhanced by previous treatment with the steroids. 

 Within 24 hours, all animals receiving saline alone succumbed; 

 pre-treatment with hormones prevented lethal effects of typhoid 

 vaccine. The lymphopenia does not appear to be altered by 

 adrenal cortical hormones, but the increase in neutrophils is 

 markedly augmented. 



538. MILLER, C. P., HAWK, W. D. and BOOR, A. K. 



Protection against bacterial endotoxins by penicillin and its 

 impurities 



Science 107:118-119, 1948 



The protective effects of penicillin against a series of endo- 

 toxins injected into the mouse was confirmed through observa- 

 tions of animals receiving both purified and impure penicillin 

 before intraperitoneal administration of endotoxin. Preparations 

 were derived from gram-negative organisms. Such protection was 

 demonstrated against the endotoxins of S. paratyphi A., S. para.^ 

 "t^yphi B., Shigella paradysenteriae , S. enterltides , S, aertrycke 

 and Aerobacter . The LD50 for mice pre-treated with penicillin 

 was three times greater when the cyrstalline product was employed 

 and nine times greater when mice were given the impure product. 

 The impure penicillin preparation lost its protective activity 

 when treated with penicillinase or heat sufficient to inactivate 

 penicillin; addition of the original amount of crystalline peni- 

 cillin restored its capacity for protection. 



539. MORGAN, H, R, 



Resistance to the endotoxins of enteric bacilli in man 



