191 



-19^8- 



545. SELIGMAN, A. M., SHEAR, M, J„, LEITER, J. and SWEET, B. 



Chemical alteration of polysacchc?.ride from Serratla maroescens . 

 I. Tumor-necrotizing polysaccharide tagged with radioactive 

 iodine 



J. Nat. Cancer Inst. 9:13-13, 1948 



Radioactive iodine was incorporated in the molecule of the 

 Shear polysaccharide and its effects oa normal and tumor- 

 bearing animals and humans were observed. Saturation of the mole- 

 cule occurred over a broad range of I concentrations; a stable io- 

 dine linkage could be demonstrated. 



When injected intravenously in dosages ranging from 12 »5 to 300 

 gammas, radioactivity disappeared rapidly from the blood stream, 

 but there was a prolonged maintenance of a residum (about 20^), 

 Greatest radioactivity was present in the liver, lung and kidney, 

 decreasing in this order, Ti-nior -necrotizing properties were not 

 affected, and little radioactivity existed in tumor tissue, 



546. TAL, C, and OLITZKI, L, 



The toxic and antigenic properties of fractions prepared from 

 the complete antigen of Shigella dysenteriae 



Jo Immunol. 58:337-348, 1948 



A series of fractions were prepare.i from the complete antigen 

 of the S strain of Shigella dysenteriaeo A phospholipid, a con- 

 jugated protein and a simple protein, undegraded and degraded 

 polysaccharides were found on fractionation , The conjugated 

 complexes contained varying amounts cf phosphorus while the 

 degraded substances were free of this elements 



These fractions were tested for their ability to produce the 

 following alterations in animals: lethal effects (mice); hypo- 

 thermia ( in mice, rats and guinea pigs); leukopenia (guinea 

 pig and rabbit); reduction of liver glycogen and adrenal ascorbic 

 acid (in rats); the Shwartzman phenomenon (rabbits) and hemor- 

 rhage production in adrenals (guinea-pig) or placentas of preg- 

 nant mice. Antigenicity of the fractions for the rabbit was 

 determined , 



The simple protein, phosphorus-froe., did not elicit the endotoxic 

 reactions produced by the conjugated complexes. The conjugates 

 reduced glycogen and ascorbic acid, and produced leukopenia 

 and hypothermia. Production of a type specific antigen followed 

 administration of the protein. The undegraded polysaccharide 

 produced the Shwartzman reaction, leukopenia and hypothermia and. 



