194 



-19 4 9 



552. ANDERSON, W. H. and BRODERSON, R. 



Hypothermia In the mouse as a bloassay of endotoxin protection 

 factor In Impure penicillin 



Proc. Soc. Exper. Biol. & Med. 70:322-325, 19^9 



Young adult mice conditioned In a constant temperature for 

 two days before and during experiments received either (1) 

 0.5 nil. Impure penicillin containing 10,000 micrograms 

 crystalline penicillin per ml. Intraperltoneally 20, l8 and 

 2 hours before Injection of 0.05 ml. of endotoxin (2) Impure 

 penicillin alone or (3) no treatment. In further experiments 

 hydrogen peroxide oxidation was used to destroy protective 

 activity, and comparisons were made with controls protected 

 by unoxldlzed materials. A constant dose of endotoxin was 

 administered to a third group; It contained various dilutions 

 of the protective factor. S. aertrycke endotoxin had the 

 ability to reduce temperature markedly, while Impure peni- 

 cillin previously Injected protected against lethal effects 

 and hypothermia. 



553. AUBERT, J. P. 



Existence of a polysaccharide In Bacillus megatherium 



Compt. rend. Soc. blol. 229:477-478, 1949 



Methods for preparing polysaccharide concentrates from B. 

 megatherium are described. Sporulatlon decreases the quanti- 

 ty obtainable. The age^^ the culture^and carbon sources In the 

 media wield Influence over the amounts produced. 



