215 



-19^9- 

 L6. WHARTON, D. R. A., and CREECH, H. J. 



Further studies of the Immunological properties of polysaccharides 

 from Serratia marcescens ( Bacillus prodigiosus ) . II. Nature of 

 the antigenic action and the antibody response In mice. 



J. Immunol. 67:135-153, 19^9 



A tolerance which reached its height on the third day after a 

 single injection of P-10 polysaccharide-lipid complex of S. 

 marcescens protected mice against a lethal dose of the poly- 

 saccharide. Heating did not diminish ability to produce anti- 

 bodies but did reduce toxic properties. The agglutinating 

 ' antibody reached high titer levels on the 10th day after admin- 

 istration of the substance. Anaphylaxis was not produced (in 

 guinea pig) . Two fractions may be present in the complex, a 

 toxic and an immunizing component. ' 



617. YAGI, Y., EGAMJ, F., HOSOYA, S., NONOBE, K., HAYASHI, T. and 

 HOMMA, Y. 



Studies on the endotoxine of Salmonella enteritidis , Gartner. 

 I. Studies on the chemical nature of the endotoxin. II. 

 Studies of the amino acid content and the sugar component of the 

 endotoxin 



Jap. J. Exper. Med. 30:2^^5-259, 19^9 



A crude endotoxin was prepared from S, enteritidis and subjected 

 to fractional precipitation. The resulting concentrate contained 

 2.9-3.6^ N, 1.1-1.58<^ P, 2.1^ S and 43-55^ reducing sugars. 

 Positive reactions to the Bial and Dische tests for carbohydrates 

 were noted. Absorption curves reached their peaks at 2400-2700 

 Angstrom units. Three fractions were secured by heating with 

 acetic acid: a neucleoprotein (MLD 1 mg.), a polysaccharide, non- 

 toxic, which contained methyl-pentose, pentose and desoxy-sugars. 

 The methyl-pentose fraction exhibited two hydrazones and a 

 phenylosazone which resembled those found in the breakdown of 

 f ucose. 



