- 1 9 5 - 



twelve intravenous injections of pyrlfer, has been free from any 

 clinical signs of chronic colitis despite two attacks of dysentery 

 In other similar cases it is said that results were somewhat com- 

 plicated by the fact that this preparation produced less fever; 

 wartime processes may have been responsible. 



657. JACOBS, P. A. 



Damage produced by Pseudomonas aeruginosa fraction in sarcoma 

 37 



Canad. Res. 10:227, 1950 



Cultures of Ps. aeruginosa R, grown on semi-synthetic media con- 

 taining glycerol and fatty acids, contained a fraction capable 

 of inducing damage to Sarcoma 37. Intraperitoneal administration 

 of fractions from either culture fluid or cell pack of the or- 

 ganism produced macroscopic hemorrhage and succeeding necrosis 

 of tumor tissue in mice. The tumor damage was confirmed micro- 

 scopically. Fractionation of the culture fluid showed that the 

 activity was resident in a water-soluble, alcohol-insoluble 

 polysaccharide. The method of measuring tumor response to 

 treatment is described. 



658. KAPLAN, M. H., COONS, A. H. and DEANE, H. W. 



Localization of antigen in tissue cells. III. Cellular distri- 

 bution of pneumococcal polysaccharides. Types II and III in the 

 mouse 



J. Exper. Med. 91:15-30, I95O 



Distribution of pneumococcal polysaccharides in the tissues of 

 the mouse was followed histochemically by means of the fluores- 

 celn-antibody technique (Coons, et al. ) or the periodic acid 

 method (Schiff; McManus). Preparation of tissues was spaced at 

 intervals ranging from 1 day to 6 months after a single dose of 

 the polysaccharide under consideration. 



Polysaccharides were found in most constant and striking concen- 

 trations in the cells of the reticuloendothelial system, the 

 capillary endothelium and in fibroblasts throughout the body. 

 They were detectable in monocytes and lymphocytes, hepatic, 

 cardiac and smooth muscle cells, in uterine epithelium, the 

 steroid -forming cells of the adrenal cortex, in testes and ovaries 

 They were persistent for 75 days in macrophages of the lymphoid 

 organs, the Kupffer cells of the liver, interstitial macrophages 

 of the myocardium, in lung septal cells, capillary endothelium 

 and renal glomeruli. After six months, they were still present 



