233 

 - 1 9 5 - 



663. MENKIN, V. 



Further studies on thermostablle leukocytosis factor of exudates 



Federation Proc. 9:338, I950 



A thermostable leukocytosis factor may be Isolated from exudates 

 which contain the pyrogenlc (thermolablle) factor. The thermo- 

 stable factor does not produce fever or leukopenia but Induces 

 leukocytosis through stimulation of the discharge of young 

 granulocytes Into, the circulation. It Is not a polypeptide but 

 contains at least one carbohydrate group. It Is believed that 

 the thermolablle leukocytosis factor may be a polypeptide. 



66^^. MTOIK, P. 



Zur Fleberbehandlung der akuten Poliomyelitis ( Pyretotherapy 

 of acute poliomyelitis) 



Therap. Gegenwart 89:274-275, 1950 



The author examines the results obtained In poliomyelitis 

 through the use of temperature-elevating substances. In his own 

 services, vaccine, pyrlfer and nucleohexyl have been utilized, 

 as reported by R. Muller and H. Arens (Therap. Gegenw. 19^7- 

 1948) . 



665. NESSET, N. M., MCLALLEN, J., ANTHONY, P. Z. and GINGER, L. G. 



Bacterial pyrogens. I. A pyrogenlc preparation from a Pseu - 

 domonas species 



J. Am. Pharm. A. (Scl. ed.) 39:456-459, 1950 



A relatively non-toxic bacterial pyrogen has been obtained from 

 a culture of a Pseudomonas organism. After growth over a period 

 ranging from one to ten days, cellular materials were digested 

 by trypsin, centrlfuged, washed and lyophlllzed. Digestion by 

 the tryptlc method employed was compared with proteolysis by 

 papain, pepsin, polldase-S, pancreatin, a fish enzyme, duodenal 

 powder, ictozyme, protease and an intestinal preparation; none 

 yielded satisfactory results. Nine enzyme preparations with 

 dlastatlc activity failed to produce detectable decreases in the 

 pyrogeniclty of cells. Other solvents and fractionation methods 

 were studied. 



Approximately 50 per cent of the concentrate obtained was pyro- 

 genlc polysaccharide. Seven per cent was hexosamine and 27 

 per cent deaoxyribonucleic acid. Ribonucleic acid was present 

 (8.1^). Protein content was negligible. The ash constituted 



