- 1 9 5 - 



virus of mlee 



Bft«t. Px>oo. (19gQ) 6tSf 



Orii«-n«CAtlve b«eillax»y eellt h«d an Inbibltory eff««t on the In- 

 flaamatory an4 lethal aetlvlty of the pnf uftonla virus when ap- 

 plied intpanasailly to mice, befors Infection or two days day 

 after. Species employed Included S. t yphosa and paratyphi , k, 

 Band C. S. t yphlmCirlum , E« coll , ?. dysenterlae (Shiga and 

 Piejaaer) and Proteus 0X19. Preparations given intraperltoneally 

 exhibited no activity. Filtrates of broth were Inactive. The 

 agent was not a polysaccharide, since a trypsin digest was like- 

 wise Ineffective. Activity of cells was not affected by aUto- 

 oalvlng, phenol or acetone ejctraction. A similarity to the 

 auclnase from V. coTOma described by Burnet Is dlscussSd. 



678. arUART, ?. a. 



Alterations In connective tissue mast cells Induced! by bact;erlal 

 pyrogens 



Am. J. Physiol. 163j754, 1^0 



In ooilnectlye tissue ijiast cells In abdominal skin of the mouse, 

 a concentration of granules around a central nucleus may be seen 

 with a few cells exhibiting some dispersion of granules when 

 cell boundaries are Indefinite. Aft^r peritoneal «»nd oral PYROMQf 

 In dosages ranging between % and 5Q giammas per kg. body weight, 

 akin biopsies were taken at "intervSls varying from 15 minutes to 

 30 ^ays after injection. A response to PYROMEN was elloltedf 

 which reached its peak In severt to i;en days after s single (5 



fammas per H|5.)dose. ''Numerleall.y, laorphologlcally and spatially 

 t followed a definite pattern dependent qn the dosage, elapsed 

 time and stra^lh of experimental nice employed" Opanules of many 

 mast cells (dispersed, the dispersion beginning in the vlolnlty 

 of small venules and capillaries beljween ^ to 15 mlhutes after 

 administration, according to Ijhe dosage given. Groups of mast 

 cells some distance from blood vesaeis were less rapidly changed; 

 there was an alteration in shape away from the ovoid* granules 

 spread out from the cell center. Many of the perlcaplllary cells 

 assumed a spindle shape. 



679. STUART, E. Q. 



The mast cell response to admiiilistratlon of a bacterial pyrogen 



J. Nat. Cancer Inst. 10:1375-1376, I95O 



After 5 to 50 gammas of PYROMpi per kg. body weight had been ad- 

 ministered to mice by intraperitoneal Injection or the oral route, 

 skin biopsies were taken at intervals between I5 minutes and 24 



