242. 



- 1 9 5 - 



were not related to dosage levels. Within 12 to 2k hours a 

 panendocrlne response was manifested with vacuolation of cell 

 cytoplasm in the suprarenal medulla, hypophyseal basophil and 

 chromophobe cells^all zones of the suprarenal cortex, the 

 parathyroid, islets of the pancreas and ovarian stroma cells. 

 Leucopenia and eosinopenia appeared within one hour and reached 

 their peak levels in four hours. Lymphocytosis followed. In 

 contrast to the action of ACTH, no suprarenal hypertrophic 

 changes were seenj in the mouse mast cells show marked granu- 

 locytic dispersion after PYROMEN. Prolonged administration 

 of PYROMEN stimulated growth of the suprarenal cortex, with in- 

 creased width of the reticularis but decreases in other zones. 

 Capsule cells appeared to be transformed to glomerulosa cells. 

 Prolonged high doses led to a reduction of anterior lobe baso- 

 phil population associated with involutional changes. 



688. WINDLE, W. P. 



Endocrine and connective tissue cellular responses to adminis- 

 tration of a bacterial pyrogen 



Abst. of Communications, XVIII Inter. Physiol. Congress (Copen- 

 hagen) Augp 15-18, 1950- p. 518 



Events following PYROMEN administration were studied in various 

 species. One to 5 gammas per kg. induced leucopenia and eosino- 

 penia within one hour in the rat, cat, dog and human. The low 

 point was reached in 4 hours, and lymphocytosis followed. In- 

 creased output of urinary corticoids was not detected in rabbits 

 and cats, but pronounced temporary increases in dogs and man 

 have been reported by others. In the mouse, connective tissue 

 mast cell granular dispersion was noted within one hour. A pan- 

 endocrine reaction to PYROMEN was seen in all organs of rabbits. 

 These changes began in 12 to 24 hours, and maximal effects were 

 present for periods up to 10 days; vacuolation of cell cyto- 

 plasm in adrenal medulla and cortex, pituitary chromophobes and | 

 basophils and parathyroid and pancreatic cells were seen. In- 

 terstitial cells of the ovarian stroma became hypertrophied with- 

 in 24 hours, with vacuolation. Prolonged administration of 

 PYROMEN created hyperactivity of lymphatic organs, inducing 

 hyperplasia and even metaphasia with granulocyte production in 

 the spleen. In all organs there was increased vascularity. 

 Proliferation of certain connective tissue cells followed. After 

 spinal cord transection in dogs and cats, fibrous and glial 

 scars appear to be inhibited while the cellular tissues develop- 

 ing between cut surfaces permit the penetration of regenerating 

 central neurons . 



689. WINDLE, W. P. and CHAMBERS, W. W. 

 Regeneration in the spinal cord of the cat and dog 



