252 



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709, GINGER, L, G., NESSET, N„ M,, RIEGEL, B, and PITZSIMONS, E. J. 



Bacterial Pyrogens, II. Pyrogenlc preparations from various 

 bacterial species 



J. Am. Pharm. A. (Scl. Ed.) 40:421-424, I95I 



Pyrogenlc concentrates, prepared from the cell bodies of five 

 bacterial species by means of tryptic digestion, were charac- 

 terized by essential chemical and biological differences. Cellu- 

 lar materials from E. coll , Pr. vulgaris , S. typhi and B. subtilis 

 were grown and collected by a method previously described (j. Am. 

 Pharm. A„ 39s456, 1950) Both cellular material and concentrates 

 were analyzed for the following constituents 1 ribonucleic acid, 

 desoxyribonuclelc acid, hexosamine, pyrogenlc polysaccharide and 

 tree lipid „ Total nitrogen, protein and nitrogenous residue were 

 f%l%3\^' '^^^ smallest content of pyrogenlc polysaccharide 

 U5oO^) was found in the concentrate from Pro vulgar is; the largest 

 content was found in the concentrate from bT subtilis. The poly- 

 saccharide content bore little relationship to biological actlvl- 

 J^i 2\u°^ active concentrate was that from S. typhi . It con- 

 rained the smallest amounts of hexosamine and unclassified reducing 

 sugars. Proteus vulgaris concentrate, with low pyrogenlc poly- 

 saccharide content possessed high biological activity. On the 

 il %Z ' ^^® concentrates prepared from B. subtilis contained 



Ojo2^ pyrogenlc polysaccharide but had the lowest biological 

 activity. 



^^i^ll^^^^^^^^ Pyogenic polysaccharide composition may account 

 a°Lf,?f/^^,^^Ji°^^i^ activity. »'The possibility that biological 

 not be ?ve?looker "^ differences in molecular structure can- 



710. GREENE, Lo Co 



Healing of thermal burns in cats treated with PYROMEN 

 Am. J. Physiol. l67;789, 1951 



thp^^,^J^f!!f^■'■°?/™° ^2 diameter, were produced on either side of 

 riS4?^Q!Sr^ ?''^™ °f the cat by the method outlined by Peters 

 ^ ?.n^^l^ZV;..J ? f^''^®^ °^ ^2 animals, intravenous injection of 

 at^seiei^i ^nllT^^^ polysaccharide (PYROMEN) were administered 

 at several dosage levels. Pour animals received initially 20 

 oinL'^fn ot P^^^^y ^teadily increasing to 60 gammas per kg. 

 8? da?s tSo «^?^^ M^i^t^^ance doses were 10 gammas per day for 



men? wSs ron?inMi;?^^%^?''^^^?? °<"2 gamma three times a day. Treat- 

 ment was continued until healing was complete. 



efanfmalf Jn^^nn^^^'^r ''°l^'^ ^^*^^^" ^^^^ appearance in the treat- 

 ed animals and controls. For the former, scab formation was rapid, 



