257 

 - 1 9 5 1 - 



719. MENCZEL, E. 



A note on the depyrogenizatlon of infusion solutions by hydrogen 

 peroxide 



J. Am. Pharm. A. 40:175-176, 1951 



The Taub and Hart method for depyrogenizing infusion solutions 

 has been slightly modified by workers of the Government hos- 

 pital, "Yarkon" , One modification replaces the Soxhlet appara- 

 tus with large pyrex boiling flasks. After boiling, contents 

 are readjusted to the original volume. The usual standard ti- 

 tration with permanganate is replaced by other methods; fair re- 

 sults were obtained with the spot test of Pelgl. The reagent 

 used, a mixture of 0.4^ ferric chloride solution and 0.8^ po- 

 tassium ferricyanide solution in equal parts, is sensitive to 

 0»08 gamma of hydrogen peroxide in a drop. Sensitivity of the 

 test may be further increased if a 10 drops per 100 cc. solu- 

 tion ratio is maintained. Dextrose causes no interference. Both 

 oxidizable substances and pyrogenic contamination may be de- 

 tected through use of the test. It may be applied to parenteral 

 dextrose solutions. Elimination of detectable hydrogen peroxide 

 by boiling with manganese dioxide for 15 minutes (0.1^) or the 

 use of activated charcoal (1^) for saline and dextrose combina- 

 tions is to be recommended. 



720. MITCHELL, S. Q., and STUART, E. G. 



Role of adrenal gland in mechanism of leucocyte changes upon 

 administration of PYROMEN 



Am. J. Physiol. l67:8lO, 1951 



PYROMEN, a purified bacterial polysaccharide, was injected intra- 

 venously into cats in dosages ranging from 0.5 to 35.0 gammas per 

 kg. body weight. The leucocyte reaction was studied in both nor- 

 mal and adrenalectoraized animals. When adrenalectomy was uni- 

 lateral, no significant changes were elicited in the leucocyte 

 counts until the second or third day after the operation when the 

 typical response was observed. The remaining gland was unable to 

 compensate fully. After bilateral adrenalectomy with maintenance 

 on DCA or cortisone, no leucocyte changes could be noted at any 

 dosage level employed. When portions of the adrenal gland were 

 transplanted into the adrenalectomized animal and desoxycortico- 

 sterone discontinued, PYROMEN again induced leucopenia on the 5th, 

 6th and 7th days; on the 8th and 9th days the initial rise in 

 leucocytes was recorded. 



