262 



- 1 9 5 1 - 

 731. STUART, E. G, 



Accelerating effect of PYROMEN in contrast to inhibitory ef- 

 fect of cortisone on the Arthus phenoraenon in rabbits. 



Federation Proc. 10 (Pt. I.) 133, 1951. Abstr. Am. J. Med. 

 Sci, 221:470, I95I 



Twenty-four male rabbits were sensitized with crystalline egg 

 albumin cutaneously; I/3 of the group received daily injections 

 of cortisone (10 mg.) intramuscularly and eight others were 

 given PYROMEN intravenously (10 gammas daily). Precipitin tests 

 for serum antibody were made at intervals. Controls (8 animals) 

 exhibited Arthus reactions in one week, pronounced on the 10th 

 day and appearing in intracutaneous sites throughout the term 

 of experimentation. After PYROMEN injections, the Arthus reac- 

 tion appeared earlier (4th or 5th days). The lesions passed 

 rapidly through the usual course, but with more intensified in- 

 flammation, hemorrhage, suppuration and necrosis. The size of 

 the area exceeded that seen in control animals. Scab formation 

 was rapid and resorption occurred, usually without sloughing. 

 Precipitin titers were noted earlier in PYROMEN-treated animals 

 and were at least 50^ above those seen in controls. "These effects 

 of PYROMEN may be correlated with its known property of stim- 

 ulating the leukopoietic, phagocytic and reticular elements of 

 the body" . 



732. STUART, E. G. 



Connective tissue mast cell responses to bacterial pyroe-ens. 

 ovalbumin and cortisone ^o- & > 



Anat. Rec. 109:351, 1951 



p^rrogens extracted from a Pseudomonas species, PYROMEN, or from 

 Proteus vulgaris and B, subtllls were injected intraperltoneally 

 into C-57 mice in dosages of 10 gammas each. Type response of 

 the connective tissue mast cells differed from that produced by 

 substances chemically unrelated. Proteins and steroids, as oval- 

 bumin and the adrenocortical factors or hormones, ACTH, DCA and 

 cortisone, were also injected in 10 mg. single doses. Only slight 



?S??n25r ''?r-,^^i?3:3^^ ^? ^^^ ^^^^^ ^^°' cortisone had a marked 

 Influence widely different from that exerted by the pyrogens. 



?h!^™''' ^ "'^- single injection, produced dissimilar effects; 

 n hi?5^?o^? concentrate from E. typhosa , (10 gammas) produced 

 ^,^i?J°^°Slc picture resembling tha^seen after ovalbumin In- 

 nfruJi^?!*o/ ^^f pyrogens used, alterations in granulation, 

 pyknosls or expansion of cells were seen. Distribution of cells 

 ^2^,""! Sroups were altered and cellular deterioration was ob- 



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