267 



- 1 9 5 2 - 



temperature from 0<,24 to lc97° C. at the single unit level of 

 dosage, and as much as 2 .,5° C. at higher dosages. The onset 

 of pyrexial response from p3n:'ogen was delayed for 1-1/2 to 

 2 hours - the exact period of the hypothermic influence of 

 ACTH, The possibility that oxytocic activity from posterior 

 lobe pituitary hormone might be responsible was ruled out 

 by careful testingo 



The significance of hypothermic effects of ACTH in relation 

 to routine testing of drugs is apparent. The ability of ACTH 

 to delay the appearance of pyrogenic responses will make 

 longer testing periods (to five hours, preferably) obligatory, 

 It is believed that the activity of ACTH in a given disease 

 condition may be wholly or in part due to nonspecific meta- 

 bolic or vascular alterations. 



7lf3, FREEMAN, G, 



The anticoagulant effect of bacterial polysaccharides in normal 

 and thrombocytopenic plasma of leukemia 



Blood, 7:235-242, 1952 



Polysaccharides from extracts of cultures of Frledlander' s 

 bacillus , type A, of Serratia roarcescens (P-25 of Shear") 

 or type II pneumococcus were compared for respective anti- 

 coagulanF~activity with a heparin sodium solution. Recalci- 

 flcation and clotting times were computed for both normal 

 and thrombocytopenic plasma „ 



An arbitrary amount of polysaccharide from Frledlander ' s 

 bacillus , type A, Inhibited retraction of the fibrin clot« 

 Pneumococcal polysaccharide has less anticoagulant activity 

 than either the Frledlander or S, marcescens somatic carbohy- 

 drate « These two materials were used in comparing recalclfl- 

 cation time when amounts of polysaccharides were Increased in 

 blood platelet ranges remained normal Comparisons vd.th 

 thrombocytopenic plasma were also made. Effective concentra- 

 tions of the polysaccharides were as low as 0,05 mg, per Opl 

 ml, normal plasma, but heparin was shown to be 400 to 1000 

 times more effective as an anticoagulant, 



in leukemic blood the two bacterial extracts had an exaggerated 

 anticoagulant action. The evidence that bacterial polysac- 

 charides exist in combination with antibody in disease states 

 is reviewed, Non-glucoseamlne polysaccharide In serum may be 

 increased to 300 mg, per cent in disease. Work in progress 

 at this time may prove more conclusively a relationship between 

 the presence of such polysaccharides, the bleeding phenomenon and 

 fever in leukemia. 



