271 



- 1 9 5 2 - 



The effect of pyrogens on capillaries, their relationships to 

 the Shwartzraan phenomenon and to tumor hemorrhagic and necro- 

 tizing changes in tumor tissue, are not, to date, fully ex- 

 plained o The alterations in blood component distribution and 

 vascular pressures are well-established. It is not yet known 

 whether or not the leucocyte response is a direct or indirect 

 phenomenon; the time required for its production after pyrogen 

 administration would suggest that some intermediary is required 

 for its appearance. 



In studies now in progress, tuberculin protein fractions have 

 been shown to have more definite effects than the polysacchar- 

 ides on the leukocytes o Fractions which produced clumping 

 of leukocytes, swelling and Inhibition of cellular migration 

 also produced fever after Intravenous injection, 



750 o SOKOLOPP, Bo, EDDY, Wo H,, CONE, G, and POWELLA, R, 



Stress reactions and tumor-breaking property of bacterial poly- 

 saccharide 



Federation Proe , 11 (Pt, I) 427, 1952 



Increased capillary fragility appears to be the prime factor 

 in the tumor-breaking property of bacterial polysaccharide. Rats 

 bearing Crocker rat carcinoma were given 0,5 mg„ per 100 Gm, 

 body weight of P-25 (Shear bacterial polysaccharide) alone or 

 with 10 mg, per 100 Gra„ body weight of flavonoid compound. When 

 the flavenoid accompanied the dose of bacterial polysaccharide 

 66 per cent survived and the remaining 3^ per cent had an ex- 

 tension of life for, on the average, 46 hours. Controls suc- 

 cumbed In approximately 7 hou-^s and 44 minutes. When the ascor- 

 bic acid level of the blood plasma was reduced to 0,01 mg, per 

 100 cc, plasma and 0,1 mg, per 100 Gm, body weight of P~25 

 Injected, the mortality was increased to 25 per cent or a 

 10 per cent increase over control groups; tu'^or destruction, 

 however, was very extensive, approximately 5o3 times greater 

 in area than in controls, 



751. TENNENT, D. M. 



Isolation of bacterial pyrogens 



Federation Proc, 11 (Pt, I) 58, I952 (Abstract) 



In a comparison of methods for isolating active pyrogenic sub- 

 stances, a crude bacterial pyrogen concentrate, prepared by pre- 

 cipitation of harvest supernatants of collform or Salmonella 

 organisms, had higher activity than that reported for purified 



