NINTH ANNUAL YEAR BOOK— PART X 485 
been tested with tuberculin and found to be free from tuberculosis before 
the inoculations were made. It is important to observe in this connec- 
tion that two out of four, or 50 per cent, of the cultures obtained from 
cases of generalized tuberculosis in children proved vrulent for cattle. 
Mohler, workng in the Patholigical Division, Bureau of Animal Indus- 
try, has obtained three very virulent cultures of tubercle bacilli from the 
human subject. A goat inoculated subcutaneously with one of these cul- 
tures died in thirty-seven days with miliary tuberculosis of the lungs in- 
volving the axillary and prescapular glands. This bacillus was obtained 
from the mesenteric gland of a boy. Of still greater interest is a bacillus 
insolated by Mohler from human sputum. A goat inoculated subcutan- 
eously with a culture of this germ died in ninety-nve days of pulmonary 
tuberculosis. A cat inoculated in the same manner died in twenty-three 
days of generalized tuberculosis. A rabbit inoculated with bovine culture 
for comparison lived ten days longer than the one inoculated with this 
sputum germ. Mohler also inoculated subcutaneously a one-year-old 
heifer with a culture derived from the tubercular mesenteric gland of a boy 
four years of age. This culture was always refractory in its growth under 
artificial conditions, and the bacilli were short, stubby rods, corresponding 
in appearance with the bovine type. At the autopsy, held one hundred 
and twenty-seven days after the inoculation, the general condition was 
seen to be poor and unthrifty, and large, hard tumors were found at the 
points of inoculation. On the rigth side the swelling measured 3VL> by 
5 inches, and the corresponding lymph gland was 2% inches long by 1% 
inches in diameter. This gland contained numerous clacareous foci; one 
of these at the apex was an inch in diameter. The lesions on the left 
shoulder of the animal were very similar to those found on the right 
side, but the dimensions of the tumor were slightly less. The lungs pre- 
sented an irregular mass of tubercular nodules, and seven or eight grape- 
like nodules were seen on the parietal pleura. Bronchial and mediastinal 
lymph glands contained numerous tubercular foci, and the pericardium, 
peritoneum, spleen, and liver were also affected. 
In order to throw some light, if possible, upon the morphological con- 
stancy of the different types of tubercle baculi, Mohler has made com- 
parative studies of bacilli from various sources, and which had been 
passed through various species of animals, by making the cultures upon 
dog serum after the method described by Theobald Smith. Some im- 
portant results have been obtained. One culture of human bacilli which 
had morphological and cultural peculiarities similar to those of the bovine 
bacillus, and which only produced local lesions in cattle, was passed 
through a series of five cats. It was then found to be completely changed 
in its morphological characters, the rods being elongated, slender, more 
or less beaded, and entirely of the human type. But far from decreasing 
in virulence, as might be expected from its morphological appearance, this 
bacillus had so increased in its pathogenic activity that it now produced 
generalized tuberculosis in a cow. This cow was inoculated subcutan- 
eously in front of each shoulder with 2 c.c. of a salt solution emulsion of 
the tuberculous omentum of the last cat of the series. The cow rapidly 
lost flesh, had a temperature of 104°P., with the point of inoculation and 
