2 s. GOTO. 



tiouing. Moreover, corro.sive sublimate can be so easily carried about, 



and its saturated solution so easily made that it is, generally speaking, 



by far the best reagent for use on a collecting tour. The specimens 



were preserved in 70 7o alcohol. 



For staining sections I have almost exclusively used Kleinen- 



berg's solution of haematoxylin. I have tried picro-carmin and 



borax-carmin, but they did not give good results, although the latter 



was very excellent for staining specimens mounted ui toto. I have 



also tried cochineal tincture so hiirhlv recommended bv Lant»- for the 



glandular cells of polyclads ; but it gave no ditferential staining 



whatever. 



For ])reparation in toto, the specimens were killed under the 

 pressure of a cover-slip over the flame of an alcohul lamp, and were 

 directly immersed in 70 "/o «'dcohol, in which they were preserved 

 together with other specimens. For staining I have used borax- 

 carmin ; the over-stain being thoroughly washed out with acidulated 

 70 -/o nicohol. In most specimens, only the internal organs and the 

 nuclei of the mesenchyma remain stained, while the mesenchyma 

 itself is wholly decolourised, so that the result forms altogether a very 

 beautiful ol)ject under the microscope. 



To Prof. Ijima and Prof. IMitsukuri are due my warmest 

 thanks both for super visicjn and for iriving: me sug-orestions and the 

 most friendly assistance. To i^rofs. Par on a and Perugia of 

 Genoa, Prof. Monticelli of Naples, and Prof. Ramsay Wright 

 of Toront(j, I am indebted for their courtesy in sending me their 

 papers on ectoparasitic Trematodes. Finally but not least my best 

 thanks are due to the authorities of the Imperial University for 

 taking charge (^f the publication of the paper. 



