20 ART. 14— K. YEXDO: 



showed that this modification was due to the parasitic .Diatom. The 

 exact determination of the parasite is beyond the present purpose. 



^ hen the section had been treated in anilin blue for 2-1 hours, 

 we gained a beautiful preparation stained metachromatically. The 

 outermost layer which fills up the intercellular space and apparently 

 takes the place of the middle lamella stains in deep purple. The next 

 layer stains in light blue. The innermost layer which stained in 

 deep violet in hematoxylin is now separable into two parts. The 

 innermost is very thin, but stains in deep purple ; the outer layer in 

 purple also but more faint. These two layers stained in the same 

 degree when treated with hematoxylin. 



Hence we might divide the celhvalls of the genicular cells into 

 four parts, with the exception of those of Amp. stelligera. 



I. The middle lamella : filling up the intercellullar spaces and 

 often hardly distinguishable at the axial portion of gcniculum by 

 virtue of the strong mutual compression of the cells : staining deep 

 in hematoxylin and anilin blue. 



This lamella corresponds in its position, especially when it is 

 very thin, to the intercellular substance of DiPPEL.^ 



II. The primary cell irai I : staining in less degree with the 

 colouring matters. With hematoxylin it stains in pale violet and 

 with anilin blue in light blue. 



III. The secondary lamella : staining in deep violet with 

 liain.'itnxylin, and in pale purple with anilin blue. It is this lamella 

 which characterises the genicular cells when stained with the colouring 

 materials. It slaines much deeper than other parts in almost all 

 colouring; reagents. 



I\. The tertiary lamella: staining in hematoxylin and anilin 



1) DlPPEL : Die Neuere Theorie über die feinere Structur der Pflanzenhülle. 



