riROPLASMA KIGEMINUM AND ANAPLASMA CENTKALE. 349 



Heifer 4154. Acted as control to 41 18, the blood being 

 inoculated within an hour of taking^. A reaction occurred 

 to P. higemiuum on 20th to 22nd day after inoculation, 

 and to A. centrale from 36th to 56th day with marked anaemia. 

 Both heifers 41 18 and 4154 were subsequently inoculated with 

 blood of heifer 3831, which had been taken and used within an 

 hour. Neither heifer gaye any further reactions. 



In this experiment P. bigeminum survived for 24 hours in 

 citrated blood, the reactions being later in both the experimental 

 animal and the control. 



Experiment 3. 



Blood from heifer 3830 was taken into citrate solution, and 

 heifer 4156 was inoculated subcutaneously with 5 c.c, kept for 

 24 hours. Heifers 4331 and 4332 acted as controls, being inocu- 

 lated with 5 c.c. each of the blood within an hour of taking. 



Heifer 4156 did not react to redwater. but did to A. centrale 

 from 38th to 51st day after inoculation. 



Heifer 4331 reacted to P. bigeminum on the 22nd day after 

 inoculation, and to A. centrale from the 45th to 51st day, the 

 reactitins in both cases being poor. 



Heifer 4332 did not react to P. bigemin\im. but a slight 

 elevation of temperature occurred on the 14th day. with subse- 

 quent slight anaemia. A very mild reaction to A. centrale took 

 place from the 44th to 50th days, only the fact that smears were 

 taken at the time the reaction Avas due causing it to be noticed 

 at all. 



Heifer 4156. When subsequently reinoculated with blood 

 of heifer 3830. within an hour after taking, the heifer reacted 

 to redwater on 13th and 14th day. No further reaction to A. 

 centraie occurred. The evidence in this experiment is not quite 

 satisfactory, as although the experimental animal reacted pro- 

 perly, one of the controls did not. The controls were imported 

 cattle, but had been in the country for some months, and although 

 stabled during the period, may possibly have been exposed to 

 infection. 



Experiment 4. 



In conjunction with experiment 2. and using the same 

 control, a heifer 4155 was inoculated with 5 c.c. citrated blood 

 of heifer 3831, which had been kept ^2 hours. 



Heifer 4155. No reaction occurred to P. bigeminum, but a 

 slight elevation of temperature took place. The reaction to A. 

 centrale was scarcely perceptible as well. When subsequently 

 inoculated with fresh citrated blood of heifer 3831, no further 

 reactions occurred. Two months later, to see what reactions the 

 blood of heifer 4155 would produce when inoculated into a sus- 

 ceptible animal, a bull 4462 was inoculated with fresh citrated 

 blood. 



Bull 4462. A temperature reaction occurred on the loth 

 day after inoculation, but P. bigeminum was not demonstrable 



