2 ART. 4. — M. SHIGA : ON THE EFFECT 



on this subject. The work has been done at the suggestion and 

 under the guidance of Prof. M. Miyoshi of the Botanical In- 

 stitute of the Science College of the Imperial University of Tokyo, 

 and I wish to express my sincere thanks to him. 



To ascertain the effect of an injury done to the root, I have 

 in all cases used water-culture ; whereas in determining the effect 

 of the removal of leaves and stems, I have experimented with 

 pot-culture. 



1. Water-culture. 



Seeds of equal size after having been soaked in water about 

 twenty- four hours, were allowed to germinate in damp sawdust, 

 and when the seedlings attained some growth, they were selected 

 and transferred to water-culture. 



Glass cylinders holding about two liters, were used for this 

 purpose. In each cylinder, I put five plants, nourislied with 

 Sachs' or Knop's solution in 0.5 7oo concentration. The cultures 

 stood in a south window, where they were left until the roots 

 had attained a sufficient length. Then, some of the latter were 

 removed with a pair of scissors. 



2. Pot-culture. 



Two kinds of pots were used, the one containing three liters 

 and the other one liter. No nourishment was supplied, except 

 the water necessary to keep the soil moist. 



Each put held five seedlings of the same growth and was 

 placed on the ground outside of the laboratory-building. 



