24 Art. 5.— Y. Toda: 



tivo protonemata with only a few spherical cells, and up in the 

 air many lateral innovations. No lateral innovations were observed 

 in the liquid. The Kxop and Detimee solutions gave the best 

 result for the growth of the moss. 



In comparing the shape of the aerial protonema with that of 

 the aquatic one, I uoticed that the filamentous cell was somewhat 

 longer and thinner, and the chlorophyll grain of the coll smaller 

 in the latter than in the former (Fig. 10). 



VIÏ. Effect of Lime on the Growth of the Moss. 



By the studies of H. Paul (5 and 6), it has been ascertained 

 that all kinds of lime salt are not equally injurious to the 

 Sphagnum, an alkaline salt of lime, such as calcium carbonate, 

 being most harmful. Boas (1) ascertained that 0.05 — 0.28^ solution 

 of calcium nitrate and of calcium chloride facilitates the growth of 

 some species of moss. We have not been told yet of any case of 

 Schisfostega growing in a calcareous cave, either in Europe or 

 in Japan. In order to ascertain whether the moss really dislikes 

 lime or not, I put the shoots of the moss in solutions of different 

 concentration, of calcium nitrate, calcium sulphate, calcium chloride, 

 and calcium carbonate, and examined their growth in the each 

 case. 



1) Calcium nitrate. — In 0.d% and 0.0 d% solutions the moss 

 produced a few adventive protonemata in 2 weeks, and in 2 

 months a few lateral innovations from its stem. The development 

 of the moss was better in a 0.05X solution than in a 0.3% one. 

 In 0.1% and 0.2% solutions, it made the greatest grow" th, bringing 

 out many adventive protonemata in 2 weeks, and prodacing numer- 

 ous lateral innovations from the stem in 2 months. The develop- 

 ment was better in a 0.1% than in a 0.2% solution. 



