Notes on a New Boveria species. .13 



granular plasm is directly, or almost directly, attached to the 

 meganuclear membrane (figs. 9, 10, and 13, N). The above con- 

 traction process of the granular plasm occurs on the outset equally 

 in both the plasmosomic and the chromatin granules (figs. 4 and 

 5). But this is not so later, when the intranuclear peripheral 

 clear space has grown wider than before (figs. G— 10, N), then the 

 peripherally situated chromatin granules cease to partake in the 

 further contraction of the plasmosomic granules, and begin to 

 liberate themselves from the last mentioned eventually to attach 

 themselves to the meganuclear membrane. Althougli it is very 

 difficult to decide if the granules so disposed represent the entire 

 chromatin in the meganucleus, yet we are inclined to believe that 

 to be the case, since in ^^reparations treated with difierential stains, 

 there exist in the central granular mass so far contracted no 

 bodies which show staining reactions for chromatin. No further 

 change takes place in the meganucleus during the process of con- 

 jugation. It never manifests any sign of disintegration, which, in 

 other infusorians, usually befalls the meganucleus at a certain 

 period of conjugation. On the contrary, in the present species, the 

 conjugant meganucleus persists in the state described above not only 

 during conjugation but even after it. In this respect the species offers 

 a remarkable exception to the general rule among the infusorians. 

 The formation of nuclei in the ex-conjugants goes on in the 

 following way. Of the four small nuclei resulting from the twice 

 repeated division of the synkarion, those three, in which the chro- 

 matin has acquired a comma-like shape (fig. 13, g. d. s//i_3), under- 

 go degenerative changes and cease to exist as independent structures, 

 but attach themselves to, and then become incorporated with the 

 meganucleus. In the meganucleus of an ex-conjugant represented 

 in fig. 14, there are found three relatively large and deeply stained 

 dots {fj. d. si/1^3), which are conspicuously larger than the ordinary 



