572 IOWA DEPARTMENT OF AGRICULTURE 



checks and no difference could be noticed as they all became sick the 

 same time and showed good lesions of hog cholera. The sodium citrate 

 enables us to save between fifty and one hundred cubic centimeters in 

 each cholera shoat by eliminating the clot, not speaking of the time saved. 

 We used five cubic centimeters of a ten per cent solution of sodium citrate 

 to one hundred cubic centimeters, and experienced no clotting, this was 

 always placed in the jar before the blood was drawn, and necessitates an 

 expert judgment as to the quantity of virulent blood the cholera shoat 

 will produce. Several tests were made using the citrate in the virulent 

 blood with no indications of reducing the virulency, but increased the 

 quantity drawn and also saved time. 



As yet we have seen no reason why it cannot be used to advantage in 

 the virulent blood. The following is one of the experiments; we could 

 quote many, but they all ran about the same and deem one sufficient to 

 illustrate our point: 



TEST ON SODIUM CITBATE IX VIRULENT BLOOD. 



Hog No. Description Date Killed 



141 Black boar, wt. 70 pounds Sept. 22, 1910 



150 White boar, wt. 60 pounds Sept. 21, 1910 



On Sept. 14, 1910, each received five cubic centimeters of virulent blood 

 subcutaneously defibrinated with sodium citrate from cholera hogs No. 

 138, pen No. 2. 



151 White sow, wt. 70 pounds Sept. 20', 1910 

 146 Black sow, wt. 60 pounds Sept. 23, 1910 



On Sept. 14, 1910, each received five cubic centimeters of virulent blood 

 defibrinated with sterile rods by beating and stirring from cholera hog 

 No. 138, Pen 2. 



In conclusion the results of the serum used by the hog raisers have been 

 all that could be expected in most cases, though in one case contrary re- 

 sults were obtained. This does not, however, offset the successes of the 

 serum treatment. 



The appropriation has been inadequate to carry out our work com- 

 pletely though we have done much good. The serum at the present price 

 of fifty cents per dose is above the reach of many farmers, and until an 

 appropriation is made to support our work on a larger scale, the price 

 should remain at fifty cents in order to make the laboratory self-sustain- 

 ing, but this deprives many hog raisers of its benefits. 



