692 CARREL— FURTHER STUDIES ON [November t,, 



from the ice-box and broken. The vessel is removed from the tube, 

 put in a jar of Locke's sokition at the temperature of the laboratory, 

 thoroughly washed and placed in warm vaseline. Afterward, the 

 vaseline is expressed from its lumen, and the segment grafted onto 

 the artery of the host. As soon as the circulation is established 

 through the artery of the host, the transplanted segment, which is 

 white, takes back immediately its normal color and becomes almost 

 similar to the other parts of the artery. Sometimes the small ves- 

 sels of the adventitia appear neatly injected with blood. In seg- 

 ments of carotid artery, preserved for eight and eleven months in 

 cold storage and grafted on the carotid of a dog, the vasa vasorum 

 were seen full of blood as soon as the circulation was reestablished. 

 The results of the transplantation of arteries, preserved in cold 

 storage, are generally excellent from a functional standpoint, even 

 if the vessel has been kept for one or two months outside of the 

 body. But, from an anatomical standpoint, the microscopical con- 

 stitution of the vessel is markedly modified when it has spent a long 

 time in cold storage. The duration of the period during which a 

 vessel can be preserved without occurrence of any lesion, is not 

 exactly determined. However, it seems that an artery, preserved 

 for more than eight days in cold storage, undergoes always, after 

 transplantation, a degeneration of its muscular fibers, while the 

 other parts of the vessel seem to remain normal. Several times a 

 perfect histological condition of the transplanted artery was ob- 

 served. A piece of carotid artery from a dog was put in a sealed 

 tube with a few drops of Locke's solution and, two days afterward, 

 transplanted onto the carotid artery of another dog. Two weeks 

 after the operation, the neck of the dog was reopened. The circu- 

 lation through the carotid was normal. The transplanted segment 

 looked like the other parts of the carotid. It was resected and 

 examined histologically. The adventitia was thickened and con- 

 tained several small vessels. The media was normal. The nuclei 

 of the muscular fibers were found entirely similar to those of a nor- 

 mal artery. The intima was well preserved and slightly thickened. 

 This observation shows, evidently, that a vessel can be preserevd in 

 cold storage and live again normally when transplanted. It is not 

 a dead, but a living artery, with all its normal anatomical elements. 



