130 Transactions of the Society. 



paper (ii)* by one of us a special text-figure has been given, and 

 the reader is referred to this.f On Plate II are figures of nerve, 

 sperm, egg, liver and gut cells, to illustrate our views. Each cell 

 ■when suitably prepared by either a Formalin-silver nitrate method, 

 or an Osmic acid technique, shows a Golgi apparatus in an excentric 

 juxta-nuclear position (PI. II, figs, lb, 2, 3, 4 and 9), or in a 

 partly diffuse (PI. II, fig. Ic), or a completely diffuse condition 

 (PI. II, figs, la and 5). Mitochondria are always found after 

 the application of certain well-known techniques, and are shown 

 in PI. II, figs. 2, 3, 8 and 9 at M. 



In growing QggB, or oocytes of animals, one finds not only 

 mitochondria and Golgi apparatus elements, but also various sorts 

 of deutoplasmic materials — yolk, fat and glycogen. 



Apart from the new structures which have been described 

 within the cell cytoplasm, the modern technique has revealed at 

 least one kind of granule or rod unknown hitherto within the 

 nucleus {36). This new intra-nuclear body has been called a 

 nucleolinus, and the recent observations of Carleton {S) have 

 revealed the fact that the nucleolinus stains differently from the 

 chromosomes, and is capable of independent binary fission. (See 



* The italic figures within brackets refer to the Bibliography at end of the 

 paper. t Journ. R. Micr. Soc, 1919, p. 96. 



EXPLANATION OF PLATE II. 



Lettering.— Gk = Golgi apparatus ; A = acrosome ; M = mitochondria ; N = 

 nucleus ; NO = nucleolus ; NL = nucleolinus ; V = vacuole (glycogen) ; Y = 

 yolk. 



Scale of figures on right bottom corner of Plate. 



Fig. 1. — Dorsal root ganglion cells of cat, to show at a, b, and c passage of 

 Golgi apparatus from an excentric juxta-nuclear position (b) to a difiuse stage (a). 

 Drawn from a Cajal preparation made by Dr. Penfield, Histology Laboratory^ 

 Oxford. 



Fig. 2. — Spermatocyte of the cavy showing- Golgi apparatus (compare with 

 fig. 16) and mitochondria. (Cajal followed by acid fuchsin.) 



Fig. 3.— Spermatid of cavy showing Golgi apparatus, mitochondria, and 

 acrosome. (Mann-Kopsch-Altmann method.) 



Fig. 4. — Spermatocyte of Limnxa showing Golgi apparatus. (Kopsch's un- 

 modified method.) 



Fig. 5. — Oocyte oiLimnxa showing Golgi apparatus i diffuse (compare with 

 fig. la) and yolk bodies. (Same method.) 



Fig. 6. — Metamorphosis of Golgi element to form a yolk body, as seen in 

 Kopsch's methods (OsO.,). Not to scale. 



Fig. 7. — Metamorphosis of mitochondrium into a yolk body, as seen in 

 Altmann's method (acid fuchsin). Not to scale. 



Fig. 8. — Binucleate liver cell of rabbit showing glycogen vacuoles and mito- 

 chondria (M). (Formalin andiron hsematoxylin.) 



Fig. 9. — Cell of mucous membrane of cat's duodenum showing Golgi apparatus 

 (GA), mitochondria (M), nucleolus (NL), and nucleolinus (NO) stained black. 

 From a preparation by Cajal's method, with safranin, made by Mr. H. M. Carleton. 

 Histology Laboratory, Oxford. 



Fig. 10.— Oocyte of Linmsea by Altmann's method ; mitochondria red, yolk 

 brownish. 



