134: Transactions of the Societij. 



AVitli regard to the use of the word Golgi apparatus, it is neces- 

 sary to point out that in young germ cells and in all embryonic 

 cells the apparatus occupies a position surrounding the archoplasm 

 and centrosome, from which the elements of the apparatus are 

 rarely separated. The word Golgi apparatus we take to mean all 

 the Golgi elements or dictyosomes (Perrincito, So) en bloc ; the 

 words Golgi element or dictyosome refers to a part of the apparatus 

 lying discrete. 



For a much fuller treatment of the subject, see the following 

 papers {11-20, 21-2 J^ and 32) in the bibliography ; also note the 

 inicrochemical reactions explained on pages 129 and 135 of the 

 p^'esent paper. 



IJitocJionchio. — Numerous grains or filaments scattered in the 

 cytoplasm, which act as follows : — 



. 1. Eedden in acid fuchsin after bichromate of potash and 

 osmic fixation (11). 



2. Stain violet by Benda's method, red by Champy-Kull, and 

 black in iron-ha-matoxylin after neutral formalin or clu'ome- 

 osmium fixation. 



3. Do not go Idack but red after Mann-Kopsch-Altmann, or 

 Kopsch-Altmann ; do not stain after Bouin or corrosive acetic, or 

 Petrunkewitsch or Carnoy fixation, followed by Ehrlich's haema- 

 toxylin or methyl-blue eosin (etc.). 



4. Stain intra vitam in Janus green and neutral red (IS), 



5. Either do not stain, or are only golden brown or greyish, 

 after Cajal's silver-nitrate method. (For further details see 

 4, 9-24, ol, 35, and ^2) 



Yolk. — The woid has been applied to any granular formations 

 of the cytoplasm of the egg. This usage must be abandoned, for 

 recent researches show that certain granules hitherto called yolk 

 are really true mitochondria, similar to those of ordinary somatic 

 cells. The g^^^^ contains yolk and mitochondria and Golgi apparatus, 

 the yolk being something different from the two latter protoplasmic 

 inclusions. It seems certain that part of both mitochondria and 

 Golgi elements can change into yolk ; this makes it difficult to 

 give a simple definition of the word. In any given species one 

 could easily draw up a table distinctly showing the difterences 

 lietween yolk and protoplasmic inclusions such as the mitochondria ; 

 but such a table would not apply to every other species, because 

 the yolk in eggs of different species, even in closely allied forms, 

 may vary greatly in its chemical constitution and origin. 



The main constituents of the yolk of the hen's Q^g, which has 

 been carefully examined, are protein, fat and lipin, and there is 

 little d<.)ubt that tlie true yolk of invertebrate eggs also consists of 

 protein, fats, and ]i]»ins, though not necessarily in the same per- 

 ci^^ntage. 



While typical yolk spheres will be found to consist of these 



