154 Transactions of the Society, 



Under this section we are obliged to conclude that the so-called 

 chromatiD emission of Schaxel is very probably a misinterpreted 

 stage in the evolution of the mitochondria, described from prepara- 

 tions made by methods which do not produce the best results. 



Such definite cases of extrusion of chromatin ic granules as are 

 known {17) do not resemble anything described by Schaxel. 



The Nucleolus and the Formation of Yolk. 



The nucleolus (plasmosome) of the germinal vesicle of young 

 o5cytes may also give rise to bodies which are themselves to be 

 considered yolk granules, or which metamorphose into yolk. 

 Hempelmann {20) and Buchner {2a), for instance, in the archian- 

 nelid Saccocirrus, describe the partial fragmentation of the young 

 oocyte nucleolus, the migration of these fragments through the 

 nuclear membrane, and the subsequent formation of yolk from 

 them. 



The secondary nuclei of insect eggs, which may originate from 

 the nucleolus do not seem to have any connexion with such bodies 

 described in Saccocirrus. 



jSTote also Schreiner's work mentioned on page 143, and Dendy's 

 observations on Grantia (5). Schaxel's descriptions and figures do 

 not coincide with Hempelmann's {:20) observations for Saccocirrus, 

 and the two processes are unalike. 



The Mitochondria and Golgi Apparatus in Human 

 Post-mortem Material. 



The cells of the human body, like those of other animals, are 

 known to contain the above-mentioned mitochondria and Golgi 

 apparatus. 



It has been found by experiment that very soon after death 

 the protoplasmic inclusions partially or wholly disintegrate, under- 

 going a sort of plasmc^lysis ; consequently post-mortem material, 

 unless procured almost immediately after death, will not be suitable 

 for a study of any of the cytoplasmic inclusions. 



It should also be pointed out that the ether or chloroform used 

 to produce anaesthesia may occasionally have an effect on the cell 

 inclusions and so introduce artifacts, especially in small animals. 



Mr. Carleton, of the Physiological Department of Oxford, 

 informs us that he has been unable to note any changes in the 

 Golgi apparatus of the gut cells of decerebrate cats, or in cats which 

 have been continuously under an anesthetic for an hour. It is 

 nevertheless necessary to avoid tissues which have been exposed 

 in any way to injurious agents. 



