ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 211 



obtained, sub-cultures in test-tubes were made with all sterile pre- 

 cautions, and when a good tube-culture bad been obtained it was used 

 for culture purposes as required. (Temperature, 15-20° 0.) 



No bacterial or other adventitious growth was obtained by sowing 

 from these successful cultures into (1) nutrient broth, (2) nutrient agar, 

 (3) glucose agar, and (4) litmus milk, at room temperature or at 36°C. ; 

 nor on the special medium itself stiffened with agar ; although pecuUar 

 rod-shaped bodies (10 /x long by 2 /x broad) were detected in growing 

 cultures with a y^^th-in. oil-immersion lens. Experiments with the 

 special medium showed that when eingle amino acids were supplied, 

 histidine, arginine, and leucine gave more rapid, growth than trypto- 

 phane ; also that galactose and fructose, but not maltose, could be sub- 

 stituted for glucose. J. E. 



p 2 



