PEOCEEDINGS OF THE SOCIETY. 251 



Dr. C. Da Fano gave a demonstration of the Gol^i Internal Appa- 

 ratus in Nervous and other Tissues. (Details of this demonstration will 

 be found on pages 157-61 of the present issue of the Journal.) 



Dr. Murray congratulated Dr. Da Fano. For himself the particular 

 interest was that although they all had a prejudice against metallic 

 impregnation methods, they must recognize that, by the application 

 of the new methods, the Golgi apparatus presented a characteristic 

 appearance in each of the tissues. Although the appearances differed 

 according to the staining method, it was very suggestive that they had 

 there a real constituent of the cell. "What its function was or what it 

 was for it was difficult to determine. 



Mr. E. J. Sheppard said that in Professor Schaefer's " Essentials of 

 Histology " was shown an enlarged figure of an animal cell in which 

 were seen the radiating structures, and these were described as tropho- 

 plasm or canula which might be demonstrated or not according to the 

 method of fixation. The canula could not be seen unless the prepara- 

 tion was suitably fixed. He had many times seen the canula in various 

 structures in the unfixed and the lightly-fixed preparations, and he was 

 not sure that he had not also seen them by the aid of dark-ground 

 illumination. He was not an upholder of the precipitate method as 

 applied to silver impregnations. If the canals did exist it was easy to 

 precipitate upon them and show the structure. If they existed there 

 was a very wonderful field for research. At present he looked upon 

 them with some scepticism. He warmly congratulated Dr. Da Fano 

 upon his beautiful exhibits. 



Dr. Gatenby thanked the Society for the interest it had shown in 

 the discussions on the Golgi apparatus and mitosis. He had attended a 

 good many meetings, and had met with a deal of scepticism. He was 

 glad to hear Mr. Sheppard now admit that there might be such a thing 

 as the Golgi apparatus. It had been seen intra vitam in tissue cultures 

 and in the ovotestis and ganglion cells of the snail. He had lately been 

 using Dr. Da Fano's method, and some of his best results had been 

 obtained with this cobalt nitrate method. 



Dr. Drew drew attention to two exhibits that were, being shown. 

 They were from an onion and a rat. The chief interest lay in the fact 

 that the specimens had been fixed by Dr. Da Fano's method, cut with a 

 freezing microtome, and stained with iron-hasmatoxylin. 



The President said that the Society was much indebted to Dr. 

 Da Fano and to the other observers who had taken part in the discussion, 

 which had brought out many important points. He moved from the 

 Chair that the best thanks of the Meeting be accorded to Dr. Da Fano. 



This was carried by acclamation. 



Mr. T. E. Wallis read a paper on " The Lycopodium Method of 

 Quantitative Microscopy," which was illustrated by lantern slides and 

 exhibits. The paper is printed in the present issue of the Journal (see 

 pages 169-78). 



A discussion followed, in which Mr. Barnard, Mr. Sheppard, and 



