INJECTION EXrERIMENTS OX PLANTS. 15 



bundles ; in this case, the transverse connections may effect that 

 conduction, for example, in the parallel-veined leaf of Rlioeo 

 discolor, provided with many delicate transverse connections among 

 the longitudinal veins, I observed that lithium was conducted 

 transversely to the margin (exp. 20, 21, PI. I, Fig. 4. A, B). 



3. Speed of conduction of the injected solution. 



A rough idea about the rapidity of movement of the injected 

 substance was arrived at in my experiments. It was ascertained 

 that the speed varied according to the substance employed ; 

 lithium was carried most rapidly, but SO^Cu and eosin less 

 rapidly, and aniline dyes the least. Indeed, aniline violet was 

 conducted so slowly that it was left just as it was in the injected 

 part, even for some hours, but was then slow^ly conducted up- 

 wards and at last so far that the extension was no longer 

 detectable. 



After some comparative experiments, I confirmed that the 

 rate of conduction of several aniline dyes (Oveeton, 8), varies 

 according to their kind, i.e. a basic aniline dye (for example 

 dahlia- violet), was less conductible than an acid one (for example 

 <3ongo-red or indigocarmine). 



The relative rapidity of movement, however, depends on the 

 concentration of the substance employed, because the proportion 

 of osmosis and diffusion may be altered by varied concentration 

 of a solution (Gaunersdoefer, 6). To ascertain this fact, I demon- 

 strated with the leaf of Fatsia japonlca, applying LiNOg (10^, 

 5^, 1%) solutions. After a certain time, the leaves were removed 

 from the stem, several deep incisions were made with a knife 

 along the petiole, in order to break the continuity of conduction, 

 then examined spectroscopically. 



