214 SUMMARY OF CURRENT RESEARCHES RELATING TO 



Fisher Bank, and three from near the Northumberland coast — was 

 undertaken with a view to ascertaining the distribution of Saccammina 

 sphserica Sars and Psammos^ohsera fusca Schulze. Mr. D. Brjce, on 

 " Five new species of Bdelloid Rotifers." Of these, four are assigned 

 to the genus Hahrotrocha^ and the fifth to CalUdina. 



B, Technique.* 

 CD Collecting- Objects, including- Culture Processes. 



Field Incubator.f — 0. Mayer describes a portable incubator, of a 

 total weight of 8540 grm. and external measurements of 46 x 31 x 12 

 cm., which has l^een employed in army manoeuvres and typhoid investi- 

 gations, and found to be of service in investigations carried out at a 

 distance from a laboratory base. It consists essentially of two tin 

 boxes, one of which fits inside the other, the intervening space being 

 filled with dry sand heated to ^jT" C, a thermometer and thermometer- 

 case, an asbestos covering, and a petroleum lamp. The figures show 

 the apparatus ready for use, and before being put together. 



Cultivation of the Malarial Parasite.J — Thomson and McLellan 

 recommend the following procedure. After careful sterilization of the 

 skin, 8 c.cm. of blood are drawn from the median basilic vein, and trans- 

 feiTed rapidly to a sterile tube containing 0*1 c.cm. of 50 p.c. dextrose 

 solution. The blood is defibrinated by stirring with a sterile glass rod, 

 and is then incubated at 08° C. The corpuscles settle, leaving clear 

 serum at the top of the fluid. The junction zone containing white and 

 a few red cells, is described as the culture layer, as multiplication of the 

 parasite is found to take place in this situation. 



Modification of the Novy-McNeal Medium for Cultivating 

 Trypanosomes.§— A Ponselle records that he has had much success in 

 cultivating Trypanosoma granulosum in the following medium : agar 

 20 grm., tap-water, 1000 c.cm. The melted agar is filtered and is dis- 

 tributed into test-tubes (2 to 3 c.cm. each), and then sterilized. After 

 cooling to about 50° C. it is mixed in the usual way with an equal 

 quantity of defibrinated rabbit's blood and then solidified in slopes. 

 The condensation water was inoculated with the pure (non-citrated) 

 blood of the eel. 



(8) Preparing- Objects. 



Washing Apparatus. || — For the removal of certain fixing reagents 

 from tissues, W. Yezierski makes use of the apparatus shown in fig. 21. 



* This subdivision contains (1) Collecting Objects, including Culture Pro- 

 cesses ; (2) Preparing Objects ; (3) Cutting, including Embedding and Microtomes ; 

 (4) Staining and Injecting ; (5) Mounting, including slides, preservative fluids, etc. ; 

 ^G) Miscellaneous. 



t Centralbl. Bakt., Ite Abt., Orig., Ixvii. (1912) pp. 398-400. 



t Brit. Med. Journ., 1913, i. pp. 130. 



§ C.R. Soc. Biol. Paris, Ixxiv. (1913) pp. 339-41. 



I! Zeitschr. wiss. Mikrosk., xxix. (1912) pp. 819-20. 



