88 Lily H. HUIE 



Out of 50 cells of this stage examinée! and drawn, there were as follows : 

 Hairs with more than 2 nucleoli 9. 

 Hairs with two nucleoli 19. 



Hairs with a single nucleolus 22. 



Of the 22 cells containing one nucleolus, 18 had erythrophilous granules 

 and crystalloids outside the nucleus; two of the cells with 2 nucleoli showed 

 them ; while the cell with 3 nucleoli only exhibited a few granules. 



In the ovaries of opening floivers the hairs are at their fullest state of 

 developement, Figs. 6-9, being often of very large size, Figs. 8 and 9. They 

 stain intensely blue, owing to their exceeding richness in protoplasm; while 

 the crystalloid bodies are conspicuous from their large size, and the brilli- 

 ancy with which they take the red dye. The crystalloids now often resemble 

 at first sight some of Zimmermann's figures of single large irregularly shaped 

 ones, but with careful focussing they are seen to consist of conglomerations 

 of smaller, generally elongated crystals, Figs. 8 and 9, cr. Long slender 

 forms also occur singly, and show in transverse section an oblique rhom- 

 boidal outline. Occasionally they are bent or curved, and not infrequently 

 one may observe them bordering a vacuole, Fig. 10. I examined and drew 

 50 hairs from the ovary of an opening fiower and of thèse there were : 

 Cells containing more than 2 nucleoli 1 . 

 Cells containing 2 nucleoli 7. 



Cells containing a single nucleolus 42. 



Ail thèse cells, except one containing 2 nucleoli, had granules and crys- 

 talloids in great profusion. The cell with 3 nucleoli has been represented 

 in Fig. 8, and two of its nucleoli are seen to be very small. 



In ferlilised ovaries, when the ovule has undergone its first division, 

 the few hairs which still retain their normal size and shape show some crys- 

 talloids; but the protoplasm and nuclei begin to exhibit the characteristic 

 affinity of degenerating cells for acid dyes-, for they stain of a purple colour, 

 Figs. 11 and 13, a. Most of the hairs, however, are entirely red, Figs. 12 and 

 13, b, contain large vacuoles in the protoplasm, fig. 12, v, and also in the 

 nucleus, nv, and possess few or no crystalloids. Some of the hairs are quite 

 withered and shrivelled up, fig. 13, b. I hâve several times observed, lying 

 in the loculi of ovaries of this âge, groups of crystalloids discharged by 

 withered hairs, fig. 13, c. Sometimes the entire shrunken hair remains in 

 close proximity to the crystalloids; at other times, as in fig. 13, only a few 

 traces of protoplasm, pr, are discernible about the group, looking as if a 

 large hair had burst. 



