86 Lily H. HUIE 



4. Transfer the slide to a vessel containing absolute alcohol 30 ce. and 

 1 per cent NaOH solution in absolute alcohol 4 drops. Wait till sections 

 are of a rust colour. 



5. Remove ail traces of NaOH with absolute alcohol. 



6. Rinse sections in ordinary water for one minute. Red clouds are 

 given off and the sections become bluish. 



7. Place slides for two minutes into water slightly acidified with 

 acetic acid. This is done to deepen and fully restore the blue colour, and 

 also to fix the eosin. 



8. Dehydrate, clear with xylol, not clove oil and mount in turpentine- 

 balsam. 



For results see Figs 1-14. 



By using simply a 1/2 0/0 watery solution of eosin, and decolourising 

 with alcoholic NaOH in the way detailed above, I succeeded in staining 

 the crystalloids, but the resuit is inferior to that produced by the contrast 

 of the double stain. Other acid dyes were tried with less success, Magdala- 

 rot (echt) giving the next best results. 



I hâve used Mann' s stain for the crystalloids in the aleurone grains of 

 the seeds oï Riciiuis, with beautiful results. I hâve also examined the tissues 

 of various plants in which Zimmermann finds protein crystalloids, both with 

 Mann's stain and with the Sâurefuchsin, with the resuit, that, whenever 

 I succeeded with the one method in demonstrating them, I succeeded also 

 with the other; and whenever I failed with the one stain, I failed equally 

 with the other. 



Comparing Zimmermann's method with the one I employed, I certainly 

 prefer the latter because of the great preciseness with which crystalloids are 

 stained. 



CYTOPLASMIC CRYSTALLOIDS OF SCILLA PATULA 

 AND HYACINTHUS SP. 



While examining the ovaries of Scilla patula with the help of the 

 Methylblau-eosin doûble-stain, my attention was arrested by numbers of 

 bright red granules and crystalloid bodies in the cytoplasm of the unicel- 

 lular hairs, that occur in considérable numbers on the placentas in this and 

 other species of Scilla. From their resemblance to many of Zimmermann's 

 figures it at once occurred to me that thèse bodies were protein-crystalloids. 

 Proceeding to test them I found, 



