84 Lily H. HUIE 



their sections. Up till now, in one instance only, that of the potato tuber, 

 hâve protein crystalloids been proved to be in the cytoplasm. Those which 

 form the subject of the présent paper are also cytoplasmic, and are found 

 in Se illa patula, etc. 



Cell-sap Crystalloids. Under this head it is convenient to group ail 

 other known instances of crystalloids occurring in the body of the cell, ( 1 1 in 

 ail, according to Zimmermann), since, with the exceptions just mentioned, 

 whenever their true position has been investigated they are said to hâve 

 been found in the Cell-sap. 



Chroma top horic Crystalloids . Schimper(i) has alreadycarefullystudied 

 the protein crystalloids of Chromatophors, and enumerated the instances of 

 their occurrences, and Zimmermann has extended his researches in this 

 direction. 



METHODS. 



Former methods. Zimmermann and Stock fix the material preferably 

 in a saturated solution of corrosive sublimate in absolute alcohol; imbed in 

 paraffin and eut sections, which vary in thickness from 5 to 10 \>.. The 

 sections are left for 24 hours or more in a 0.2 0/0 solution of Sâure-fuchsin 

 in distilled water; washed out in running water, until sufficiently decolou- 

 rised ; dehydrated with absolute alcohol, cleared up in xylol, and mounted 

 in xylol-balsam. 



Though both histologists hâve endeavoured to find other methods for 

 the successful démonstration of thèse bodies, they hâve not succeeded in 

 doing so, and corne to the conclusion that Sâure-fuchsin acts as a spécifie 

 stain. 



My methods. The ovaries of Scilla patula were fixed by me in three 

 différent ways : 



1. In absolute alcohol. 



2. In Mann's Picro-corrosive Alcohol (2), which consists of a saturated 

 solution of HgCb and Picric acid in absolute alcohol. 



3. In Mann's Watery Corrosive Fluid (3). 



(1) Schimper : Untersuchungen ûber die Chlorophyllkûrper, und die ihnen homologen Gcbilde ; 

 Pringsheim's Jahrbuch, Bd. 16, p. i. 



(2) Mann : Trans. Bot. Soc. Edin., vol. XVIII, pp. 429, 432, 1S90. 

 13) Journ. Scot. Micros. Soc, 1894, p. i55. 



