DEPARTMENT OF MARINE BIOLOGY. 217 



BLENNIIDiE. 

 Rupiscartes macclurei sp. nov. 



Head 4.2; depth 4.3; dorsal XI, 20, or XII, 19=41; anal 22. Head slightly longer than 

 deep; anterior profile vertical, slightly convex. Pectorals reach to vent or beyond in male, 

 not quite to vent in female. Upper Up of male large; teeth pectinate; canines large; fringe 

 around upper and lower lips, upper pigmented, lower not pigmented. Supraocular tentacle 

 slender; large tentacle medial to each anterior nostril, which divides into 5 or 6 tentacles; 

 two nucal tentacles in male, smaller in female. Numerous large pores on head, one group 

 behind and below eye, another on supraopercular region. Dorsal fin entire, extending from 

 nape to caudal; last dorsal ray bound down for two-thirds of its length; last anal ray free. 



Differs generally from R. atlanticus in coloration. Color brownish generally, livid purplish 

 on costal region; filaments above eyes and edges of lips bright orange. Iris slaty. Dorsal 

 ohve-greenish, with median dusky lengthwise band anteriorly, contrasting strongly with 

 whitish or pale upper haK of fin, but sloping up behind so that edge of dorsal is dusky pos- 

 teriorly. Upper front edge of dorsal broadly orange below, with submarginal area of whitish. 

 Caudal olivaceous, dusky medianly, grayish below, yellowish above; anal dark neutral tint, 

 edge blackish; pectoral pale orange, red on lower half; ventral pale. 



Two specimens from dead coral reef west of Guanica Harbor. 



Length, 5.5 cm. 



Type, No. 3081, Zoological Museum, Princeton University. Paratype, No. 3027, Zoo- 

 logical Museum, Princeton University, 5 cm. long. 



(Named for Professor C. F. W. McClxire, for his researches upon the lymphatics of fishes.) 



Further Studies on COi in Sea Water and CO2 Production in Tropical Marine 



Animals, by Shiro Tashiro. 



The work done this year at the Marine Biological Laboratory at Tortugas 

 between July 2 and August 2 is a continuation of the researches begun last 

 summer. The original plan was to investigate the effects of temperature on 

 the metabolism of tropical marine animals and their isolated tissues, but this 

 was somewhat modified on account of the interest which has been aroused by 

 the question of "free C02"in the sea-water, which, at the request of Dr. Mayer, 

 I have again attempted to investigate. 



^Phenolphthalein-sea water; prepared by heating sea-water to 85° C. with crystals of phenol- 

 phthalein, then adding an equal volume of sea-water and again heating to 90° C. 



In spite of the presence of a certain amount of free CO2 in the distilled water 

 used, and enough free OH ions present in the sea-water to turn phenolphthalein 

 pink, tube B is much pinker than tube A. The question arises whether this is 

 due to effects of electrolytes on phenolphthalein, or the effects of electrolytes on 

 the condition of free H and OH in sea-water. If there is free CO2 in sea-water, 

 the dissociation of H2O in the sea-water must be entirely different from that 

 found in pure water. 



If we now add N/100 NaOH to tube A, until it matches the color of tube B, 

 we must have neutrahzed free CO2, if the original pale color of tube A was 

 due to the presence of free CO2 in the sea-water; but if one now adds 1 c.c. 

 excess of N/100 NaOH to the distilled water in B, it requires 7 c.c. more N/100 

 NaOH to cause the color of A to equal the intensity of B. This ratio 1 : 7 is 

 fairly constant until it suddenly breaks at the point where the NaOH causes a 



