346 MACDOUGAL AND SPOEHR— GROWTH AND IMBIBITION. 



Agar and gelatine were dissolved in the usual way and the tem- 

 perature of the suspension allowed to fall to a point below 40° C. 

 before the protein was stirred into it. In the course of the cooling 

 and drying, cloudy masses became visible which were taken to be 

 the globulin component of the protein. The dried sheets came down 

 to a thickness of .3 to .4 mm. Calibrated samples were tested in trios 

 under the auxograph in the usual manner. Two complete series of 



Fig. 10. Auxographic record of swelling of agar 90 — protein 10, sections 

 .25 mm. in thickness, in NaOH N/ioo, A = 220 per cent., in HCl N/100, 

 5 = 360 per cent., and in distilled water, C = 800 per cent. X 10. 



all mixtures were made and an additional measurement of the action 

 of water and alkali was obtained. The swellings were as follows 

 (Fig. 10) : 



Water. HCl N/ioo. NaOH N/ioo. 



Gelatine go — Protein 10 (Phaseolns). 

 585.7 per cent. 1401.0 per cent. 942.8 per cent. 

 1200.0 704.3 

 800.0 



1300.5 



817.7 



Averages : 940.0 



Gelatine 75 — Protein 25 (Phaseolits) . 

 818.1 621.2 



1060.6 848.4 



939-4 7348 



Agar go — Protein 10 (Phaseolus) . 



50.0 150.0 



75-0 150.0 



62.5 150.0 



Agar gg — Protein i {Phaseolus). 



300.0 220.0 



360.0 240.0 



330.0 230.0 



