120 



HEPBURN— BIOCHEMICAL STUDIES OF 



TABLE VI. 

 Digestion of the Protean of Castor Bean Globulin by the Pitcher Liquor. 



Liquor from 



Volume 



of 



Pitcher 



Liquor, 



C.c. 



Digestion. 



Non-stimulated pitchers. 



0.6 

 2.5 



2.0 

 2.0 



Stimulated pitchers. 



2-5 



0.5 



4.0 



No digestion at end 

 of 14 days 



Protean completely 

 dissolved in 14 

 hours 



Protean almost 

 completely dis- 

 solved in 29 hours 



Protean completely 

 dissolved in 48 

 hours 



Digestion of pro- 

 tean marked on 

 3d day, advanced 

 on 7th, almost 

 complete on 12th 

 day 



Digestion of pro- 

 tean marked on 

 4th day, almost 

 complete on 9th 

 day 



of protean, derived from the ricin, separated on the addition of the 

 acid. By the action of a protease, this precipitate was gradually 

 digested or dissolved. 



In one experiment, liquor from a non-stimulated pitcher was 

 used ; the cloudy precipitate was partially dissolved in two days, but 

 had not entirely disappeared at the end of seven days.' In another 

 experiment, liquor from a stimulated pitcher was used, the cloudy 

 precipitate was markedly digested in two days. Therefore, liquor 

 from both non-stimulated and stimulated pitchers exerted a pro- 

 teolytic action on the protean. 



Cleavage of Glycyltryptopiiane. 



Liquor from stimulated pitchers was permitted to act on glycyl- 

 tryptopiiane in the' presence of toluene as a bactericide ; lo c.c. of 

 pitcher liquor and 2 c.c. of an aqeous solution of the dipeptide (so- 

 called Ferment diagnosticum) were used. In one experiment, 



