118 



HEPBURN— BIOCHEMICAL STUDIES OF 



TABLE IV. 



Time Required for Digestion of 0.2 Gram of Carmine Fibrin 

 BY THE Pitcher Liquor. 



The results of both series of experiments (Tables III. and IV.) 

 demonstrate that the liquor from both non-stimulated and stimu- 

 lated pitchers dissolved carmine fibrin in the presence of 0.2 per 

 cent, hydrochloric acid, and that liquor from non-stimulated pitchers 

 had absolutely no digestive action on that substrate in the absence 

 of that acid. 



Digestion of Edestan. 



The solution of edestan was prepared by dissolving' o.i gram of 

 edestin in 15 c.c. of o.i N hydrochloric acid, previously diluted with 

 water to a volume of 25 c.c. The details of the various experi- 

 ments are given in Table V. After incubation of the mixture of 

 pitcher liquor and edestan solution, it was rendered neutral to phe- 

 nolphthalein by addition of o.i A'' sodium hydroxide solution. 



In the experiments with liquor from stimulated pitchers, no 

 precipitate formed in the determination proper on neutralization, 

 showing that the digestion of the protean edestan had advanced 

 beyond the metaprotein stage. In the experiments with liquor 

 from non-stimulated pitchers, a precipitate always formed in the 



